Deconfounding Phenology in SPAD-Based Rice Nitrogen Diagnosis Using Physiological Time and Canopy-Stratified...

Phenology can confound rice nitrogen diagnosis based on SPAD readings because leaf greenness and nitrogen concentration change nonlinearly with development. We tested whether physiological time, expressed as growing degree days (GDD), can reduce this developmental bias and improve the portability of SPAD-based diagnosis. We analyzed 1141 observations from 20 independent field experiments across five sites, spanning japonica, indica, and hybrid cultivars and nitrogen fertilizer treatments (0-300 kg N ha^-1). SPAD was measured on up to five leaf-from-top positions (LFT1-LFT5) and used to predict leaf nitrogen concentration (LNC), plant nitrogen concentration (PNC), and nitrogen nutrition index (NNI). Across group-wise cross-validation by experiment, adding GDD to SPAD consistently improved cross-environment accuracy (mean R² up to 0.75 for LNC and 0.79 for PNC) and markedly weakened residual trends along GDD. Multiplicative SPAD×GDD degraded performance, while explicit interaction terms provided little gain over a simple additive SPAD + GDD form. Interpretable analyses further showed that diagnostic information is concentrated in mid-canopy leaves and shifts with physiological time. Combining GDD with a two-leaf SPAD protocol retained most accuracy for concentration targets, supporting a time-aligned and field-practical approach for robust nitrogen diagnosis.