Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

22.0K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
22.0K
Transgenic Plants02:50

Transgenic Plants

8.8K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
8.8K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.2K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.2K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.7K
Overview
80.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical and high-resolution magnetic resonance imaging-based prediction of ischemic stroke in cervical artery dissection.

Frontiers in neurology·2026
Same author

Design, synthesis, and biological evaluation of novel selenium-matrine hybrid compounds as topo I -targeted anticancer agents.

Bioorganic chemistry·2026
Same author

Engineering Guide RNAs for CRISPR-Based Biosensors.

ACS sensors·2026
Same author

Silicon Alleviates Drought Stress and Enhances Rice Seedling Establishment Under Simulated Dry Direct Seeding via Regulation of ABA and JA Signaling.

Plants (Basel, Switzerland)·2026
Same author

Ultrasound-programmable tumor extracellular vesicles delivery system for dual immune-epigenetic therapy against colorectal cancer metastasis.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

The GSK3/SHAGGY-like OsGSK3 phosphorylates and inhibits phase separation of OsFCA at Ser-43 and Ser-45 to regulate brassinosteroid signaling and rice architecture.

The New phytologist·2026

Related Experiment Video

Updated: Feb 28, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.6K

Multiplex Gene Editing Creates Triple-Resistant Rice Against Both Insect Herbivores and Pathogens.

Guannan Qin1,2, Quanlei Shentu1,2, Jingling Pan1,2

  • 1State Key Laboratory of Agricultural and Forestry Biosecurity, Key Laboratory of the Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Plants (Basel, Switzerland)
|February 27, 2026
PubMed
Summary
This summary is machine-generated.

Researchers engineered rice with enhanced resistance to pests and diseases by editing susceptibility genes. This multiplex gene editing approach offers a sustainable strategy for developing resilient rice varieties.

Keywords:
bacterial blightbrown planthoppergene editingricerice blast

More Related Videos

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

13.3K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

5.2K

Related Experiment Videos

Last Updated: Feb 28, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.6K
Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice
07:43

Agrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice

Published on: October 6, 2020

13.3K
Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
06:37

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

5.2K

Area of Science:

  • Plant Science
  • Genetics
  • Agricultural Biotechnology

Background:

  • Rice production is threatened by biotic stresses like brown planthopper, rice blast, and bacterial blight.
  • Developing resistant cultivars is crucial for sustainable agriculture.
  • Disrupting susceptibility genes offers broader, durable resistance than race-specific genes.

Purpose of the Study:

  • To engineer broad-spectrum resistance in rice against insect pests and pathogens by simultaneously editing key susceptibility genes.
  • To assess the efficacy of multiplex CRISPR/Cas9 editing in conferring resistance without compromising agronomic traits.

Main Methods:

  • Utilized multiplex CRISPR/Cas9 gene editing technology.
  • Simultaneously disrupted susceptibility genes: ACS2 (brown planthopper), Bsr-D1/ERF922/Pi21 (blast), and Xa5 (bacterial blight).
  • Generated and analyzed three triple-mutant rice lines (abx, aex, apx).

Main Results:

  • Successfully generated three triple-mutant rice lines.
  • All mutant lines showed significantly enhanced resistance to brown planthopper, blast, and bacterial blight.
  • Agronomic traits were not compromised in the mutant lines compared to wild-type rice.

Conclusions:

  • Multiplex susceptibility gene editing is a feasible and precise strategy for rice improvement.
  • This approach enables the development of rice varieties with synchronized, broad-spectrum resistance to multiple pests and diseases.
  • Offers a sustainable method for breeding resilient rice cultivars.