Energy Metabolism and Auxin Signaling Disruption Underlying Stamen Identity Defects in Tobacco Cytoplasmic Male...

Cytoplasmic male sterility (CMS) provides a natural model for studying nuclear-cytoplasmic interactions, although the details of nuclear-cytoplasmic communication remain poorly understood. In this study, transcriptomic and metabolomic data were integrated to elucidate the molecular and metabolic regulatory networks underlying stamen developmental defects in the tobacco CMS K326 (Nicotiana tabacum). Disrupted energy metabolism, auxin pathways, and floral development gene expression were identified in CMS K326. Metabolites such as glucose-6-phosphate, fructose-6-phosphate, and oxalosuccinic acid decreased, while an accumulation of succinate was observed and auxin IAA was deficient. Our study revealed that disrupted nuclear-cytoplasmic interactions in CMS K326 are associated with concurrent disruptions in early auxin homeostasis and energy metabolism, which collectively lead to the disturbance of the stamen development program. This study provides multiomics-level evidence for understanding stamen identity defects in CMS.