Pathogenic Species of Botryosphaeriaceae Involved in Tree Dieback in an Urban Forest Affected by Climate Change

Urban forests are highly valued for the multiple benefits they provide to city dwellers. The strategic provision of ecosystem services by these forests is threatened by climate change, warming conditions being responsible for heat waves and chronic droughts that inflict stress and mortality on trees. A three-year study (2011-2013) conducted at Parco Nord Milano (PNM) (Milano, Italy) assessed the impact of thinning interventions on the dynamics of fungal pathogens in declining forest plots. Symptomatic trees of the genera Alnus, Acer, Fraxinus, Platanus, Quercus and Ulmus, exhibited in thinned subplot pronounced decline/dieback, exhibiting symptoms like microphyllia, leaf yellowing, leaf shedding, sunken cankers, shoot wilting and branch dieback. Comparative analyses between the thinned and unthinned subplots revealed a significantly higher incidence of pathogens in the thinned one. Five species of Botryosphaeriaceae, namely Botryosphaeria dothidea, Diplodia corticola, Diplodia seriata, Dothiorella omnivora and Neofusicoccum parvum, were consistently isolated from tissues of declining hosts. There is evidence that thinning altered plot-level microclimate conditions and microbial equilibrium, favoring the proliferation of latent, pathogenic Botryosphaeriaceae. In fact, during the study period, the presence of N. parvum increased tenfold and that of B. dothidea fivefold in thinned subplot. Conversely, in unthinned subplot, the same pathogenic taxa maintained stable proportions. These results demonstrate that thinning altered ecological balances increasing tree susceptibility to harmful, cosmopolitan botryosphaeriaceous fungi. Our findings challenge assumptions about thinning as a universally beneficial practice, emphasizing the need for silvicultural strategies that take into account host and pathogen ecology and the microclimatic resilience of forest stands. This study emphasizes the importance of adaptive management in urban forestry to mitigate the unintended ecological consequences of climate change.