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Inhibition of Cdk Activity

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Related Experiment Video

Updated: Feb 28, 2026

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

Published on: February 16, 2015

HTD1265 Disrupts GimC-Dependent Cellular Processes in Saccharomyces cerevisiae.

Kaori Itto-Nakama¹, Naoya Hosoyamada¹, Shinsuke Ohnuki¹

¹Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwano-ha, Kashiwa 277-8562, Chiba, Japan.

Pathogens (Basel, Switzerland)

|February 27, 2026

Summary

The antifungal compound HTD1265 disrupts GimC-dependent pathways, affecting fungal cell structure and growth. This mechanism offers a new strategy for combating challenging non-albicans Candida species.

Keywords:

Candida krusei GimC (prefoldin)Saccharomyces cerevisiae antifungal agents

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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

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Published on: September 26, 2025

Area of Science:

Mycology
Molecular Biology
Cell Biology

Background:

Candida krusei is a challenging non-albicans fungal pathogen.
Understanding antifungal mechanisms is crucial for developing new treatments.

Purpose of the Study:

To elucidate the mechanism of action of the novel antifungal compound HTD1265.
To investigate HTD1265's effects on fungal cellular processes.

Main Methods:

Integrative phenotypic analysis in Saccharomyces cerevisiae.
High-resolution morphological profiling.
Pathway inference and genetic validation.

Main Results:

HTD1265 treatment caused nuclear positioning defects and impaired mitotic spindle elongation.
The compound induced hallmarks of GimC (prefoldin) deficiency.
HTD1265 demonstrated antifungal activity by disrupting GimC-dependent processes, not directly targeting tubulin.

Conclusions:

HTD1265 targets GimC-dependent cellular processes, highlighting cytoskeletal and cell wall regulation as a fungal vulnerability.
HTD1265 serves as a valuable tool for studying GimC-dependent pathways in fungi.