Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Initiation of Translation02:33

Initiation of Translation

39.7K
Initiating translation is complex because it involves multiple molecules. Initiator tRNA, ribosomal subunits, and eukaryotic initiation factors (eIFs) are all required to assemble on the initiation codon of mRNA. This process consists of several steps that are mediated by different eIFs.
First, the initiator tRNA must be selected from the pool of elongator tRNAs by eukaryotic initiation factor 2 (eIF2). The initiator tRNA (Met-tRNAi) has conserved sequence elements including modified bases at...
39.7K
Leaky Scanning02:28

Leaky Scanning

5.8K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.8K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

11.1K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.1K
Stringent Response in E. coli01:23

Stringent Response in E. coli

419
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
419
Improving Translational Accuracy02:07

Improving Translational Accuracy

15.3K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
15.3K
The Unfolded Protein Response01:37

The Unfolded Protein Response

6.6K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tailoring texture for dysphagia diets: Unraveling the synergistic role of protein and blended vegetable oil in softening starch gels.

Carbohydrate polymers·2026
Same author

The CP312R protein of African swine fever virus inhibits host protein translation via the BiP/PERK/eIF2α pathway.

Veterinary research·2026
Same author

RNA-binding protein tristetraprolin inhibits Th2 cell activation and differentiation in allergic rhinitis by promoting TRIM18 mRNA decay.

The Journal of biological chemistry·2026
Same author

Structural Evolution From Starch-Protein Interactions Driving Hydration Behavior and Textural Changes in Steamed Sponge Cake Matrices.

Journal of food science·2026
Same author

Oil-phase modulated molecular assembly in rice bran protein fibril-chitin nanofiber emulsion gels: Structural design strategy for synchronized fat replacement and sodium release optimization.

Food research international (Ottawa, Ont.)·2026
Same author

Gastrointestinal and Colonic Metabolism of Different Types of Pea Proteins: Analysis of Structures and Metabolites.

Journal of agricultural and food chemistry·2025
Same journal

Correction: Bulatov et al. Camelpox Virus in Western Kazakhstan: Assessment of the Role of Local Fauna as Reservoirs of Infection. <i>Viruses</i> 2024, <i>16</i>, 1626.

Viruses·2026
Same journal

Correction: Franco et al. Whole Blood Volume-Based Absolute Quantification of HTLV-1 Proviral Load: A Comparative Method Evaluation Study. <i>Viruses</i> 2026, <i>18</i>, 580.

Viruses·2026
Same journal

Correction: Medkour et al. Adenovirus Infections in African Humans and Wild Non-Human Primates: Great Diversity and Cross-Species Transmission. <i>Viruses</i> 2020, <i>12</i>, 657.

Viruses·2026
Same journal

Burden of Malaria and Dengue Across Global, Asian, and Chinese Populations Based on GBD 2021 Data: A Quantitative Assessment of Importation Risks to China.

Viruses·2026
Same journal

First Report of <i>Orthonairovirus songlingense</i> in <i>Haemaphysalis concinna</i> Ticks from Russia.

Viruses·2026
Same journal

Epidemiological and Virological Characteristics of H9N2 Avian Influenza Virus in Jiangsu Province, China, 2024.

Viruses·2026
See all related articles

Related Experiment Video

Updated: Feb 28, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.4K

ASFV MGF110-7L Inhibits eIF4G1 Expression via Endoplasmic Reticulum Stress to Block Host Translation.

Xinyu Gao1, Suduo Jiang2, Liyan Zhang1

  • 1College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.

Viruses
|February 27, 2026
PubMed
Summary
This summary is machine-generated.

African swine fever virus (ASFV) protein MGF110-7L inhibits host translation by degrading eIF4G1 via stress granules and autophagy. This reveals a new ASFV immune evasion strategy and potential antiviral targets.

Keywords:
African swine fever virusendoplasmic reticulum stresseukaryotic initiation factors 4G1host translation hijackingthe multigene family 110-7L

More Related Videos

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
10:59

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Published on: May 19, 2014

18.9K
Xenopus laevis as a Model to Identify Translation Impairment
10:24

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

11.2K

Related Experiment Videos

Last Updated: Feb 28, 2026

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells
08:47

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

3.4K
Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
10:59

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Published on: May 19, 2014

18.9K
Xenopus laevis as a Model to Identify Translation Impairment
10:24

Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

11.2K

Area of Science:

  • Virology
  • Molecular Biology
  • Cellular Biology

Background:

  • African swine fever virus (ASFV) is a lethal virus requiring host translation for replication and immune evasion.
  • The ASFV MGF110 gene family has members with unknown functions.
  • Understanding ASFV's host interaction mechanisms is crucial for developing antiviral strategies.

Purpose of the Study:

  • To investigate the role of ASFV MGF110-7L in regulating host cell translation.
  • To elucidate the molecular mechanisms by which MGF110-7L affects protein synthesis.
  • To identify potential targets for ASFV intervention.

Main Methods:

  • Ribopuromycylation assays to measure nascent polypeptide synthesis.
  • Western blotting to assess protein and mRNA levels of translation factors.
  • Inhibitor assays (ISRIB, bafilomycin A1) to probe signaling pathways.
  • Co-immunoprecipitation to study protein interactions.

Main Results:

  • MGF110-7L potently inhibited host translation in a dose- and time-dependent manner.
  • MGF110-7L selectively reduced eIF4G1 protein levels post-transcriptionally, independent of mRNA changes.
  • ASFV MGF110-7L induced ER stress, promoted stress granule formation, and led to eIF4G1 degradation via autophagy, involving eIF4G1-G3BP1 interaction.

Conclusions:

  • ASFV MGF110-7L suppresses cap-dependent translation by promoting autophagic degradation of eIF4G1 through stress granules.
  • This represents a novel ASFV mechanism for controlling host translation and evading immune responses.
  • These findings offer insights into ASFV-host interactions and suggest potential antiviral targets.