Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inhibitors of Bacterial DNA Synthesis01:28

Inhibitors of Bacterial DNA Synthesis

130
Bacterial pathogens depend on precise and efficient DNA replication to sustain infection. Two type II topoisomerases—DNA gyrase and topoisomerase IV—are critical to this process, as they resolve DNA supercoiling and unlink chromosomes during replication. Fluoroquinolones, synthetic derivatives of quinolones, exploit this mechanism by stabilizing the transient DNA–enzyme cleavage complex, preventing strand religation, and causing lethal double-strand breaks. These...
130

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biological Activity of Copper(II) and Palladium(II) Complexes with a Tetradentate S,O-Donor Ligand.

International journal of molecular sciences·2026
Same author

Anti-staphylococcal potential of montanine-type derivatives from amaryllidaceae alkaloids alone and in combination with antibiotics.

Journal of applied microbiology·2026
Same author

Urate as a CO<sub>3</sub><sup>•-</sup> Scavenger and Regulator of SOD-1 and OGG1 Enzymes: Insights from DFT, Molecular Docking, and Molecular Dynamics.

Antioxidants (Basel, Switzerland)·2026
Same author

Design, Synthesis, and Evaluation of a Novel Phenanthrene Derivative as a Potential DNA Intercalator.

ACS chemical biology·2026
Same author

Palladium(II) Complexes with Chloro-Substituted Salicyl Schiff Bases: Exploring Multimodal Anticancer Mechanisms and Catalase Inhibition.

Molecules (Basel, Switzerland)·2026
Same author

Host soluble plasma factors increase dual-species Staphylococcus epidermidis and Candida albicans biofilm biomass without enhancing stress tolerance.

Scientific reports·2026

Related Experiment Video

Updated: May 3, 2026

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of GoldIII
08:26

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of GoldIII

Published on: August 31, 2018

7.3K

Polynuclear Silver(I)-Quinoxaline Complex: Comprehensive Structural Characterization, Antimycobacterial Properties

Ghada Bouz1,2, Nevena L Stevanović3, Marta Počkaj4

  • 1Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, 500 03 Hradec Králové, Czech Republic.

Pharmaceutics
|February 27, 2026
PubMed
Summary

A novel silver(I) complex with N-(3'-phenylpropyl)quinoxaline-2-carboxamide (pqx-2ca) shows potent antimycobacterial activity. This compound targets both DNA and essential mycobacterial proteins, offering a dual mechanism for drug development.

Keywords:
DNA interactionN-substituted quinoxaline-2-carboxamidesantimycobacterial activityprotein interactionsilver(I) complexes

More Related Videos

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.9K
Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms
03:29

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Published on: May 31, 2024

996

Related Experiment Videos

Last Updated: May 3, 2026

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of GoldIII
08:26

Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of GoldIII

Published on: August 31, 2018

7.3K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.9K
Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms
03:29

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Published on: May 31, 2024

996

Area of Science:

  • Coordination Chemistry
  • Medicinal Chemistry
  • Biophysics

Background:

  • Silver(I) complexes with aromatic heterocyclic ligands exhibit significant antimicrobial properties.
  • These properties are linked to their ability to interact with biological targets.
  • Aromatic heterocyclic ligands are crucial components in developing novel antimicrobial agents.

Purpose of the Study:

  • Synthesize and characterize a new polynuclear silver(I) complex with N-(3"-phenylpropyl)quinoxaline-2-carboxamide (pqx-2ca).
  • Evaluate the antimycobacterial activity of the synthesized complex.
  • Investigate the molecular mechanisms underlying the complex's activity, including DNA and protein interactions.

Main Methods:

  • Single-crystal X-ray diffraction for structural confirmation.
  • Spectroscopic techniques (NMR, IR, UV-Vis) for characterization.
  • Density Functional Theory (DFT) calculations and spectral simulations for solution behavior analysis.
  • Antimycobacterial activity assays against various Mycobacterium species.
  • Molecular docking studies to assess binding to DNA, bovine serum albumin (BSA), and mycobacterial targets (InhA, MmpL3).

Main Results:

  • Successfully synthesized and structurally characterized the polynuclear silver(I) complex [Ag(NO3)(pqx-2ca)]n.
  • The complex exhibited significantly enhanced antimycobacterial activity against Mycobacterium tuberculosis H37Ra, M. smegmatis, M. aurum, M. avium, and M. kansasii compared to the free ligand.
  • Experimental and docking studies confirmed stable binding to the minor groove of DNA and subdomain III of BSA.
  • Docking revealed inhibitory potential against InhA and MmpL3 proteins, with binding affinities comparable to standard inhibitors.

Conclusions:

  • The synthesized silver(I) complex [Ag(NO3)(pqx-2ca)]n demonstrates potent antimycobacterial efficacy.
  • The complex possesses a dual mechanism of action, targeting both DNA and essential mycobacterial proteins.
  • [Ag(NO3)(pqx-2ca)]n is a promising candidate for developing novel silver-based antimycobacterial agents.