Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Oogenesis

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

Hormonal Control of the Ovarian Cycle

Hormonal Control of the Ovarian Cycle

The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dietary <i>Bacillus subtilis</i> Improves Growth Performance, Digestive Enzyme Activity, Antioxidant and Inflammatory Responses, and Gut Microbiota in Juvenile GIFT (<i>Oreochromis niloticus</i>).

Animals : an open access journal from MDPI·2026

Same author

Development and validation of an ultrasound-based predictive model for central lymph node metastasis in papillary thyroid carcinoma with peripheral calcification.

Gland surgery·2026

Same author

The predictive value of mid-trimester baPWV combined with sFlt-1/PlGF for the adverse pregnancy outcomes of pregnant women with chronic hypertension: a prospective cohort study.

BMC pregnancy and childbirth·2026

Same author

Development and validation of machine learning models for obstructive sleep apnea risk stratification in high-risk pregnant women.

BMC pregnancy and childbirth·2026

Same author

A multienzyme-mimicking nanoplatform induces disulfidptosis/cuproptosis/apoptosis for tumor therapy.

National science review·2026

Same author

Dietary <i>Sporolactobacillus laevolacticus</i> Improves Growth Performance, Intestinal Health, and Immune-Antioxidant Related Responses in Juvenile Coho Salmon (<i>Oncorhynchus kisutch</i>).

Microorganisms·2026

Search research articles

Related Experiment Video

Updated: Feb 28, 2026

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Published on: January 4, 2017

DEHP Exposure Affects Mitochondrial Function During Ovarian Follicle Development.

Shu-Jie Yang¹, Xiao-Kang Lu¹, Li-Shu Li¹

¹College of Animal Science and Technology & College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, Zhejiang, China.

Environmental Toxicology

|February 27, 2026

Summary

Di(2-ethylhexyl) phthalate (DEHP) exposure harms female fertility by disrupting ovarian development and function. This study reveals DEHP impairs mitochondrial dynamics and DNA integrity via specific molecular pathways.

Keywords:

DEHP mitochondria mitophagy ovary oxidative stress

More Related Videos

Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

Published on: April 10, 2021

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

Published on: October 15, 2016

Related Experiment Videos

Last Updated: Feb 28, 2026

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Studying Mitochondrial Structure and Function in Drosophila Ovaries

Published on: January 4, 2017

Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

Visualizing the Effects of Oxidative Damage on Drosophila Egg Chambers using Live Imaging

Published on: April 10, 2021

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

In Situ Labeling of Mitochondrial DNA Replication in Drosophila Adult Ovaries by EdU Staining

Published on: October 15, 2016

Area of Science:

Reproductive Toxicology
Environmental Health
Molecular Biology

Background:

Di(2-ethylhexyl) phthalate (DEHP) is a widespread environmental contaminant linked to female reproductive issues.
Previous studies indicate DEHP exposure depletes ovarian follicles and disrupts hormone production.
The precise molecular mechanisms underlying DEHP's impact on ovarian function remain largely unknown.

Purpose of the Study:

To investigate the in vivo effects of DEHP on female mouse ovarian development and function.
To elucidate the molecular pathways involved in DEHP-induced ovarian damage.

Main Methods:

An in vivo mouse model was exposed to 200 mg/kg DEHP for 28 days.
Ovarian development, follicle counts, mitochondrial dynamics, oxidative stress, and DNA damage were assessed.
Key signaling pathways including Sirt3/Sod2 and Akt/mTOR were analyzed.

Main Results:

DEHP exposure inhibited ovarian development and follicle maturation, reducing primary and antral follicle numbers.
Mitochondrial dynamics were disrupted, leading to mitophagy and autophagy.
Oxidative stress, abnormal mitochondrial energy metabolism, DNA damage, and apoptosis were induced by DEHP.
Inhibition of Sirt3/Sod2 and Akt/mTOR signaling pathways was observed.

Conclusions:

DEHP exposure significantly impairs female ovarian function and reproductive capacity.
The detrimental effects are mediated by the inhibition of Sirt3/Sod2 and Akt/mTOR signaling pathways.
This research provides critical insights into the molecular mechanisms of DEHP toxicity in the female reproductive system.