Related Experiment Video
Updated: Feb 28, 2026
13:27
High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
Published on: August 8, 2019
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Vector-free DNA transfection by nuclear envelope mechanoporation
Leyla Akh1, Apresio K Fajrial2, Sunwoo Sohn2
1Biomedical Engineering Program, University of Colorado, Boulder, CO 80309, USA. xiaoyun.ding@colorado.edu.
Lab on a Chip
|February 27, 2026
Summary
This study introduces a novel microfluidic device using nanolancets for efficient genetic engineering. It enables direct nuclear delivery of plasmid DNA, enhancing cell treatment applications with high throughput and viability.
Area of Science:
- Biotechnology
- Cell Biology
- Genetic Engineering
Background:
- Genetic engineering holds promise for treating incurable diseases.
- Current nucleic acid delivery methods face challenges with carrier-induced toxicity and efficiency.
- Efficient delivery of plasmid DNA to the cell nucleus is crucial for genetic engineering.
Purpose of the Study:
- To develop a novel mechanical method for efficient and safe nucleic acid delivery into the cell nucleus.
- To overcome limitations of existing chemical and viral gene delivery systems.
- To demonstrate the potential of this technology for rapid production of genetically engineered cells for therapeutic applications.
Main Methods:
- A microfluidic device with integrated high aspect ratio nanostructures (nanolancets) was developed.
- The device was used to rupture cell membranes and nuclear envelopes for direct cargo delivery.
- The delivery efficiency, cell viability, and plasmid DNA expression were assessed across various cell types.
Main Results:
- The nanolancet device enabled repeatable rupture of cell and nuclear envelopes for direct cargo delivery.
- High cell viability was maintained across different cell types after cargo delivery.
- Direct nuclear delivery of naked plasmid DNA resulted in rapid gene expression, comparable to microinjection but with higher throughput.
Conclusions:
- The developed microfluidic device offers an efficient and high-throughput method for genetic engineering.
- This technology facilitates direct nuclear delivery of nucleic acids, improving gene expression speed and efficiency.
- The device shows significant potential for applications in regenerative medicine and treating genetic disorders.
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