Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microtubules in Signaling01:22

Microtubules in Signaling

2.2K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
2.2K
Mechanism of Ciliary Motion01:05

Mechanism of Ciliary Motion

5.4K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
5.4K
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

4.0K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
4.0K
Cell Polarization by Rho Proteins01:21

Cell Polarization by Rho Proteins

3.9K
Cell polarity is the asymmetric distribution of cellular and membrane components, making one side of the cell different from the other. This polarity is essential to many processes such as embryogenesis, axon migration, glucose transport across epithelial cells, and directional cell migration. A migrating cell responds to intracellular or extracellular signals via molecular cascades that reorganize the actin cytoskeleton to establish this polarity. In these cells, the Rho family proteins Cdc42,...
3.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peer-delivered combined EMD and imagery rescripting for traumatized Chinese university students: a RCT.

European journal of psychotraumatology·2026
Same author

Compound Heterozygous ATM Variants Cause Adolescent-Onset Cerebellar and Extrapyramidal Disease Without Telangiectasia in a Consanguineous Pakistani Family.

Genetics research·2026
Same author

Regulating Interfacial Water Distribution and Ion Transport by Rigid-Flexible Dual-Piperidinium Ionomer toward Enhanced CO<sub>2</sub> Electrolysis.

Journal of the American Chemical Society·2026
Same author

KLF4/FOXF1 axis alleviates silicosis fibrosis by modulating fibroblast behavior and extracellular matrix remodeling.

Journal of environmental sciences (China)·2026
Same author

Differentiating benign from malignant pulmonary nodules in the context of bronchiectasis: a retrospective study.

Annals of medicine·2026
Same author

A homozygous splice-site variant in <i>ZMYND10</i> causes primary ciliary dyskinesia with primary infertility in a Chinese family.

Frontiers in medicine·2026

Related Experiment Video

Updated: Feb 28, 2026

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.9K

Ccdc57 regulates cilia and left-right patterning in Xenopus.

Binyi Yang1,2, Emily K Mis2, Xianglin Zhou1

  • 1Department of Pulmonary and Critical Care Medicine, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.

Biology Open
|February 27, 2026
PubMed
Summary

CCDC57 regulates ciliary function crucial for left-right asymmetry during embryonic development. Mutations in CCDC57 are linked to laterality disorders, impacting cilia structure and function.

Keywords:
Xenopus tropicalisCiliopathyHeterotaxyLeft-right organizerLeft-right patterning

More Related Videos

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea
07:07

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

Published on: February 21, 2016

11.0K
Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
14:08

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

15.9K

Related Experiment Videos

Last Updated: Feb 28, 2026

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.9K
Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea
07:07

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea

Published on: February 21, 2016

11.0K
Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
14:08

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

15.9K

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Left-right (LR) asymmetry is established by ciliary fluid flow in the left-right organizer (LRO).
  • Disruptions in LR patterning cause laterality disorders, including situs inversus and congenital heart defects.

Purpose of the Study:

  • To identify novel regulators of ciliary function and LR patterning.
  • To investigate the role of CCDC57 in embryogenesis and its potential link to human laterality disorders.

Main Methods:

  • Depletion of ccdc57 using morpholino oligonucleotides (MOs) in Xenopus embryos.
  • Analysis of ciliary structure and function in multiciliated cells.
  • Assessment of LR marker gene expression (dand5, pitx2c) and cardiac looping.
  • Functional validation of patient-derived CCDC57 variants in a Xenopus model.

Main Results:

  • CCDC57 depletion caused abnormal cilia and misexpression of LR markers in Xenopus.
  • Cardiac looping defects were observed in ccdc57-depleted embryos.
  • Patient-derived CCDC57 variants failed to rescue ciliary defects, unlike wild-type CCDC57.

Conclusions:

  • CCDC57 is a critical regulator of ciliary function essential for LR patterning.
  • CCDC57 dysfunction is implicated in human laterality disorders, highlighting its role in embryonic development.