Associations of Endothelial-Related lncRNAs with Early-Onset STEMI: A Case-Control Study

Area of Science:

• Cardiology
• Molecular Biology
• Biomarker Discovery

Background:

• Early-onset ST-segment elevation myocardial infarction (STEMI) in individuals under 50 is a growing clinical concern.
• Improved early diagnostic markers are crucial for timely intervention in young STEMI patients.
• Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cardiovascular diseases.

Purpose of the Study:

• To investigate the potential of specific endothelial-related lncRNAs (MANTIS, NORAD, RNCR3, SENCR) as diagnostic biomarkers for early-onset STEMI.
• To evaluate the expression levels of these lncRNAs in young STEMI patients compared to age-matched controls.

Main Methods:

• A case-control study involving 200 young STEMI patients and 200 healthy controls.
• Real-time quantitative polymerase chain reaction (RT-qPCR) was used to measure lncRNA expression in blood samples.
• Statistical analysis, including Area Under the Curve (AUC) calculation, was performed to assess diagnostic accuracy.

Main Results:

• Significantly lower expression levels of NORAD and MANTIS were found in STEMI patients compared to controls (p < 0.001).
• MANTIS demonstrated high diagnostic potential with an AUC of 0.92, while NORAD showed moderate potential with an AUC of 0.68.
• No significant differences in SENCR and RNCR3 expression were observed between the groups.

Conclusions:

• Downregulation of MANTIS and NORAD suggests their potential utility as novel biomarkers for early-onset STEMI.
• MANTIS, in particular, may aid in risk stratification and guide treatment strategies for young STEMI patients.
• Further validation studies in larger, diverse populations are recommended to confirm these findings.