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Direct Imaging of Laser-driven Ultrafast Molecular Rotation

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Published on: February 4, 2017

Quantum Cascade Laser-Based Vibrational Circular Dichroism Imaging for Chiral Biosensing.

Michael Le¹, Viviana Arrunategui Norvick¹, Laurence Nafie²

¹Department of Electrical Engineering, Colorado School of Mines, Golden, Colorado, USA.

Annual Review of Analytical Chemistry (Palo Alto, Calif.)

|February 27, 2026

Summary

Quantum cascade lasers (QCLs) enhance vibrational circular dichroism (VCD) spectroscopy for high-resolution chiral imaging. This breakthrough enables detailed analysis of biomolecules and pharmaceuticals, advancing chiral sensing applications.

Keywords:

chiral biosensing chirality pharmaceutical drugs proteins quantum cascade lasers vibrational circular dichroism imaging

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Area of Science:

Chiroptical spectroscopy
Molecular spectroscopy
Biophysical chemistry

Background:

Vibrational circular dichroism (VCD) probes molecular chirality using differential infrared light absorption.
Quantum cascade lasers (QCLs) offer high-power, narrowband mid-infrared sources, significantly boosting VCD sensitivity and speed.
Previous VCD techniques lacked the resolution and speed for advanced imaging applications.

Purpose of the Study:

To review the instrumental design of QCL-based VCD imaging systems.
To demonstrate the application of QCL-VCD imaging for spatially resolved chiral biosensing.
To highlight the potential of QCL-VCD for analyzing biological and clinical samples.

Main Methods:

Utilizing QCLs for high-power, narrowband mid-IR light generation.
Implementing polarization-modulation strategies for enhanced VCD signal detection.
Developing micrometer-resolution mapping techniques for VCD imaging.

Main Results:

QCL-VCD imaging achieves unprecedented sensitivity and speed.
Spatially resolved detection of protein secondary structures is demonstrated.
Enantiomeric purity of pharmaceuticals and pathological tissue features can be visualized without labels.

Conclusions:

QCL-VCD imaging is a powerful tool for chiral analysis in biological and clinical settings.
Overcoming challenges like birefringence and data processing is key for robust imaging.
Future integration with nonlinear chiroptical techniques promises further advancements.