Related Experiment Video
Updated: Mar 1, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Dual-channel single-spectrometer chromatic confocal coherence sensing to mitigate bandwidth compression in film
Abstract:
A dual-channel single-spectrometer chromatic confocal coherence sensor is proposed for simultaneous measurement of film thickness and refractive index. Compared with the conventional single-channel single-spectrometer approach, the proposed method converts the multiplicative coupling between interferometric and confocal signals into an additive form by introducing an additional detection channel and eliminating the need for an external reference arm. As a result, the optical thickness resolution is improved by one order of magnitude. For validation, a sensor system is established, and film specimens with different materials and thicknesses are measured. The results demonstrate that the dual-channel configuration introduces no additional structural complexity while improving the optical thickness resolution from 50 µm to 5 µm, with film thickness and refractive-index measurement errors less than 11 µm and 0.02, respectively.
Related Concept Videos
Confocal Fluorescence Microscopy
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

