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Updated: Jul 20, 2026

Mimicking and Measuring Occlusal Erosive Tooth Wear with the "Rub&Roll" and Non-contact Profilometry
Published on: February 2, 2018
Effects of Experimental Polymer-Based Fluoride Varnishes Against Erosive Tooth Wear
Objectives:
The addition of film-forming polymers to fluoride varnishes may enhance their protective effect against erosive tooth wear. This study evaluated experimental varnishes combining fluoride with polymethacrylate copolymers for controlling enamel wear under erosive-abrasive conditions.
Methods And Materials:
Polished bovine enamel specimens were demineralized (0.3% citric acid, pH 2.6, 5 minutes) and assigned to six groups (n = 15) based on treatment: amino methacrylate copolymer (AMC); methyl methacrylate copolymer (MMC); AMC with sodium fluoride (AMC+NaF); MMC with sodium fluoride (MMC+NaF); ultrapure water (negative control, NC); and a commercial varnish (positive control; Duraphat, Colgate-Palmolive Company). Experimental varnishes contained 15% polymer, and fluoride-containing groups had 5% NaF. Specimens underwent nine-day erosive-abrasive cycling consisting of five minutes immersion in 0.3% citric acid, 60 minutes in artificial saliva, repeated four times daily, combined with toothbrushing for 15 seconds twice daily. Surface loss (SL) was measured after treatment to confirm varnish thickness standardization and at the end of cycling using contact profilometry. KOH-soluble fluoride (µg/cm2) on enamel was also quantified. Data were analyzed using One-way ANOVA for surface loss, and Kruskal-Wallis and Dunn post hoc tests for KOH-soluble fluoride (α = 0.05).
Results:
AMC+NaF, MMC+NaF, and the commercial varnish (Duraphat) significantly reduced SL compared to the negative control and polymer-only varnishes (p = 0.001). No significant differences were observed between Duraphat and AMC+NaF (p = 0.139) or MMC+NaF (p = 0.166). For KOH-soluble fluoride, AMC+NaF showed lower values than MMC+NaF (p = 0.001), while Duraphat did not differ significantly from either.
Conclusions:
Varnishes formulated with polymethacrylate copolymers and sodium fluoride provided in vitro protection against enamel erosive-abrasive wear, with efficacy comparable to the commercial varnish tested.
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