Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

9.0K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
9.0K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

8.9K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.9K
Abnormal Proliferation02:23

Abnormal Proliferation

5.3K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.9K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.9K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.1K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multidisciplinary Treatment of Post-radiation Sarcoma in the Left Axilla Following Radiotherapy for Breast Cancer: A Case Report and Review of the Literature.

Cureus·2026
Same author

Targeting Mouse Double Minute 2 Homolog (MDM2) Oncogene in Breast Adenocarcinoma.

Maedica·2026
Same author

Impact of C-Fos Overactivation on Laryngeal Squamous Cell Carcinoma Biological Parameters.

Maedica·2026
Same author

Epidemiological Profile and Survival Outcomes of Laryngeal Cancer in Western Greece: A 21-Year Retrospective Cohort Study.

Journal of clinical medicine·2026
Same author

Diagnostic and Clinical Significance of the Special AT-Rich Sequence-Binding Protein 2 Deregulation in Gastrointestinal Malignancies.

Maedica·2026
Same author

B-Cell Lymphoma 2 Mitochondria-Mediated Apoptotic Pathway in Cervical Carcinoma.

Maedica·2026

Related Experiment Video

Updated: Mar 1, 2026

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

1.5K

FAS/FAS-ligand Apoptotic Complex Deregulation in Laryngeal Squamous Cell Carcinomas.

Michail Athanasopoulos1, Vasileios Papanikolaou2, Dimitrios Roukas3

  • 1Department of Otorhinolaryngology, University General Hospital of Patras, Patras, Greece; miathanasopoulos@gmail.com.

Anticancer Research
|February 27, 2026
PubMed
Summary

Altered co-expression of FAS and FAS-ligand (FAS-L) is common in laryngeal squamous cell carcinomas (LSCCs). This dysregulation negatively impacts apoptosis and is linked to aggressive tumor phenotypes.

Keywords:
FASFASLapoptosiscarcinomaimmunohistochemistryprotein

More Related Videos

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex
07:17

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex

Published on: August 2, 2021

3.0K
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

114.0K

Related Experiment Videos

Last Updated: Mar 1, 2026

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

1.5K
Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex
07:17

Measuring Composition of CD95 Death-Inducing Signaling Complex and Processing of Procaspase-8 in this Complex

Published on: August 2, 2021

3.0K
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

114.0K

Area of Science:

  • Oncology
  • Cell Biology
  • Immunology

Background:

  • The FAS/FAS-ligand (FAS-L) system is crucial for apoptosis and immune regulation.
  • FAS/FAS-L activation can trigger caspase-mediated apoptosis, relevant to conditions like cataract formation.
  • Understanding FAS/FAS-L co-expression is vital in cancer research.

Purpose of the Study:

  • To investigate the role of altered FAS and FAS-ligand co-expression in laryngeal squamous cell carcinomas (LSCCs).
  • To analyze the correlation between FAS/FAS-L expression levels and clinicopathological features of LSCCs.

Main Methods:

  • Immunohistochemistry (IHC) was used to analyze FAS/FAS-L expression in 50 LSCC tissue sections.
  • Digital image analysis (DIA) was employed to quantify FAS/FAS-L expression levels.
  • Statistical analysis was performed to correlate expression with tumor stage and size.

Main Results:

  • A significant negative correlation was observed between FAS and FAS-L expression (p<0.001).
  • Higher FAS expression was associated with lower FAS-L expression.
  • FAS expression was highest in stage IV, FAS-L in stage III LSCCs (not statistically significant).
  • Tumor size significantly correlated with FAS/FAS-L expression (p=0.001, p=0.011), with larger tumors showing higher FAS and smaller tumors higher FAS-L.

Conclusions:

  • Deregulation of the FAS/FAS-L apoptotic system is frequent in LSCCs.
  • Altered FAS/FAS-L co-expression negatively affects normal apoptotic mechanisms.
  • This dysregulation is associated with aggressive LSCC phenotypes, including advanced stage and larger tumor volume.