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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Enhanced urine glucose sensing using two-dimensional TMDCs-based SPR biosensor.

Biswajit Dey1, Md Tawabur Rahman2, Arnab Saha1

  • 1Department of Electrical and Electronic Engineering, Khulna University of Engineering & Technology, Khulna, 9203, Bangladesh.

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|February 27, 2026
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Summary
This summary is machine-generated.

A novel surface plasmon resonance (SPR) biosensor using two-dimensional (2D) transition-metal-dichalcogenides (TMDCs) enhances urine glucose detection sensitivity. The MoS₂-based sensor offers accurate, label-free monitoring for diabetic and non-diabetic individuals.

Keywords:
Bi-MetallicBiosensorFDTDSurface plasmon resonanceTMDCsUrine glucose

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Materials Science

Background:

  • Surface plasmon resonance (SPR) sensing is vital for biomedical applications.
  • Traditional SPR sensors face limitations in sensitivity for accurate detection.
  • Developing enhanced SPR biosensors is crucial for improved diagnostic capabilities.

Purpose of the Study:

  • To propose and optimize a bi-metallic/2D Transition-Metal-Dichalcogenides (TMDCs)-based SPR biosensor.
  • To enhance sensitivity and accuracy for urine glucose detection.
  • To investigate the role of different TMDCs materials in SPR sensing performance.

Main Methods:

  • Utilized the Lumerical Finite-Difference-Time-Domain (FDTD) platform for sensor design and simulation.
  • Incorporated various TMDCs (WS₂, WSe₂, MoS₂, MoSe₂, MoTe₂) in a bi-metallic structure (CaF₂-Ag-Al).
  • Analyzed electric field distribution and refractive index changes for performance evaluation.

Main Results:

  • The optimized CaF₂-Ag-Al-MoS₂ structure demonstrated superior sensitivity.
  • Successfully detected urine glucose concentrations across relevant ranges for non-diabetic and diabetic individuals.
  • Achieved high sensitivity (455.83 deg/RIU), accuracy (1.32), and quality factor (110.10 RIU⁻¹).
  • TMDCs integration significantly enhanced sensitivity due to optical absorption and surface-to-volume ratio.

Conclusions:

  • The developed SPR biosensor with MoS₂ offers a promising approach for sensitive and accurate urine glucose detection.
  • The sensor provides a label-free, cost-effective method for non-invasive glucose monitoring.
  • This technology has the potential for widespread application in diabetes management and diagnostics.