Related Experiment Video
Updated: Mar 1, 2026
Murine Model of Allergen Induced Asthma
Published on: May 14, 2012
Therapeutic potential of Abelmoschus esculentus seed extract in asthmatic mice: Immunological analysis and
Suleman Hussain Shah1, Ghulam Murtaza1, Mubashar Hussain1
1Department of Zoology, University of Gujrat, Gujrat, Punjab, Pakistan.
Background:
Seeds from Abelmoschus esculentus have strong anti-inflammatory properties and may effectively treat inflammatory diseases.
Objective:
This study explores the therapeutic potential of A. esculentus seed extract on bioactive components, hematological parameters, liver function tests, leukocytes, cytokines and histology in asthmatic mice.
Methods:
Mice were divided into six groups: The normal group, groups treated with A. esculentus (10, 15 and 20 mg/mL), a dexamethasone group (3 mg/mL) and an asthmatic control group. On days 1 and 14 after acclimation, all groups except the normal control received intraperitoneal injections of ovalbumin, followed by oral doses of A. esculentus (10, 15 and 20 mg/mL) or dexamethasone (3 mg/mL) during 15th to 26th day of the experiment. All treated groups were exposed to ovalbumin inhalation for three days afterward.
Results:
The bioactive components in the acetonic extract were identified through Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The primary bioactive compounds identified were fatty acids, flavonoids and phenolics. Mice given the extract showed improved liver enzyme levels, reduced leukocyte infiltration, alleviated airway constriction, lower cytokine levels (IgE, IL-4, IL-5, IL-10, IL-13 and IL-17A) and normalized blood indices.
Conclusion:
In conclusion, the seed extract of A. esculentus has significant potential to reduce asthma symptoms in the asthmatic mouse model.
Related Concept Videos
01:20Asthma: Pathogenesis and Management
Asthma is classified as allergic and non-allergic. Allergens such as dust mites, pollen, and pet dander trigger allergic asthma, while factors like cold air, intense emotions, or exercise can induce non-allergic asthma.
01:25Antiasthma Drugs: Mast Cell Stabilizers and Anti-IgE Drugs
Mast cell stabilizers, such as cromolyn (also known as sodium cromoglycate) and nedocromil (Tilade), are effective drugs in asthma management. These stabilizers hinder histamine release by skillfully obstructing the activation of mast cells and other cellular entities. Notably, they navigate this task without...