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Two new compounds from Atractylodes japonica Koidz. ex Kitam.

Mei Yi1,2, Jing Wang1,2, Jie Sun1,2

  • 1Ministry of Education, Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Harbin, China.

Natural Product Research
|February 28, 2026
PubMed
Summary

Two new compounds isolated from *Atractylodes japonica* Koidz. ex Kitam. demonstrated significant cytotoxicity against various cancer cell lines, including HepG-2, A549, HeLa, BGC-823, and HT-29. These findings highlight potential new cancer therapeutics derived from natural sources.

Keywords:
Atractylodes japonica Koidz. ex Kitamcytotoxic activitysesquiterpenoid glycosidesstructural elucidation

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Area of Science:

  • Natural Product Chemistry
  • Medicinal Chemistry
  • Pharmacology

Background:

  • *Atractylodes japonica* Koidz. ex Kitam. is a plant used in traditional medicine.
  • Natural products are a rich source of potential therapeutic agents.
  • Cytotoxicity of plant-derived compounds against cancer cell lines is an active area of research.

Purpose of the Study:

  • To isolate and characterize new compounds from *Atractylodes japonica*.
  • To evaluate the cytotoxic activity of isolated compounds against cancer cell lines.
  • To identify potential anticancer agents from natural sources.

Main Methods:

  • Extraction of compounds from *Atractylodes japonica* using dichloromethane.
  • Structure elucidation using 1D and 2D NMR spectroscopy and high-resolution mass spectrometry.
  • Cytotoxicity assays against HepG-2, A549, HeLa, BGC-823, and HT-29 cancer cell lines.

Main Results:

  • Two new compounds, (1S,5S,7R,10S)-10-hydroxyl-deoxysecoatractylo-δ-lactone-11-O-β-D-glucopyranoside (1) and (3R,5S,10S)-Atracty-lenolide I-3-O-β-D-apiopyranosyl(1→6)-β-D-glucopyranoside (2), were isolated and characterized.
  • Compounds 1 and 2 exhibited significant cytotoxicity against HepG-2 cancer cells (IC50 values of 44.34 ± 1.79 and 46.27 ± 1.46 μM, respectively).
  • Compound 1 showed cytotoxicity against A549 and HeLa cells (IC50 values of 30.54 ± 1.58 and 36.73 ± 1.19 μM, respectively), while compound 2 was effective against BGC-823 and HT-29 cells (IC50 values of 32.53 ± 1.13 and 35.16 ± 1.78 μM, respectively).

Conclusions:

  • The study successfully isolated and identified two novel compounds from *Atractylodes japonica*.
  • The isolated compounds possess significant cytotoxic properties against a panel of human cancer cell lines.
  • These findings suggest that *Atractylodes japonica* is a promising source for the development of new anticancer drugs.