Two new compounds from Atractylodes japonica Koidz. ex Kitam

Area of Science:

• Natural Product Chemistry
• Medicinal Chemistry
• Pharmacology

Background:

• *Atractylodes japonica* Koidz. ex Kitam. is a plant used in traditional medicine.
• Natural products are a rich source of potential therapeutic agents.
• Cytotoxicity of plant-derived compounds against cancer cell lines is an active area of research.

Purpose of the Study:

• To isolate and characterize new compounds from *Atractylodes japonica*.
• To evaluate the cytotoxic activity of isolated compounds against cancer cell lines.
• To identify potential anticancer agents from natural sources.

Main Methods:

• Extraction of compounds from *Atractylodes japonica* using dichloromethane.
• Structure elucidation using 1D and 2D NMR spectroscopy and high-resolution mass spectrometry.
• Cytotoxicity assays against HepG-2, A549, HeLa, BGC-823, and HT-29 cancer cell lines.

Main Results:

• Two new compounds, (1S,5S,7R,10S)-10-hydroxyl-deoxysecoatractylo-δ-lactone-11-O-β-D-glucopyranoside (1) and (3R,5S,10S)-Atracty-lenolide I-3-O-β-D-apiopyranosyl(1→6)-β-D-glucopyranoside (2), were isolated and characterized.
• Compounds 1 and 2 exhibited significant cytotoxicity against HepG-2 cancer cells (IC50 values of 44.34 ± 1.79 and 46.27 ± 1.46 μM, respectively).
• Compound 1 showed cytotoxicity against A549 and HeLa cells (IC50 values of 30.54 ± 1.58 and 36.73 ± 1.19 μM, respectively), while compound 2 was effective against BGC-823 and HT-29 cells (IC50 values of 32.53 ± 1.13 and 35.16 ± 1.78 μM, respectively).

Conclusions:

• The study successfully isolated and identified two novel compounds from *Atractylodes japonica*.
• The isolated compounds possess significant cytotoxic properties against a panel of human cancer cell lines.
• These findings suggest that *Atractylodes japonica* is a promising source for the development of new anticancer drugs.