Two new compounds from Atractylodes japonica Koidz. ex Kitam

Two new compounds, (1S,5S,7R,10S)-10-hydroxyl-deoxysecoatractylo-δ-lactone-11-O-β-D-glucopyranoside (1) and (3 R,5S,10S)-Atracty-lenolide I-3-O-β-D-apiopyranosyl(1→6)-β-D-glucopyranoside (2), were isolated from the CH₂Cl₂ extract of Atractylodes japonica Koidz. ex Kitam., along with fifteen known compounds (3-17). Their structures were elucidated by 1D NMR spectroscopy (¹H-NMR,¹³C-NMR), 2D NMR experiments (HMBC, HMQC, ¹H-¹H COSY, NOESY), together with high-resolution mass spectrometry. The two compounds exhibited cytotoxicity against the HepG-2 cancer cell line, with IC₅₀ values of 44.34 ± 1.79 and 46.27 ± 1.46 μM, respectively. In addition, compound 1 was evaluated for its cytotoxic activity against the A549 and HeLa cell lines, with IC₅₀ values of 30.54 ± 1.58 and 36.73 ± 1.19 μM, respectively; compound 2 showed strong effects against the BGC-823 and HT-29 cell lines, with IC₅₀ values of 32.53 ± 1.13 and 35.16 ± 1.78 μM, respectively.