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Updated: Mar 1, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Interpreting Microbiome Signatures with MicrobiomeNet
Yao Lu1,2, Khoi Nguyen Nguyen1,2, Jianguo Xia1,3
1Department of Microbiology and Immunology, McGill University, Montreal, Canada.
None:
MicrobiomeNet (https://microbiomenet.com) is a web-based platform developed to provide functional insights into microbiome signatures using genome-scale metabolic models (GEMs). It currently hosts 12,400 GEMs and around 6 million microbial signatures. Users can start by searching microbes, metabolites, genes, or enzymes, and perform common tasks such as to characterize the metabolic capacity for a given microbe, to explore known microbial associations, as well as to understand potential metabolic interactions. This book chapter provides practical, step-by-step instructions for navigating MicrobiomeNet to obtain functional insights into individual microbes or microbial association networks. © 2026 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Characterizing the Metabolic Profile of a Microbe of Interest Basic Protocol 2: Elucidating Metabolic Interactions from Microbial Associations Basic Protocol 3: Analyzing Carbohydrate-Utilization Pathways to Explain Co-Responsive Taxa Basic Protocol 4: Identifying Novel Deoxycholic Acid-Producing Gut Microbes Basic Protocol 5: Assessing the Faecalibacterium prausnitzii-Coprococcus Relationship.
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