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Updated: Jun 9, 2026

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
Published on: May 19, 2018
Protocol for generating megakaryocytes from patient induced pluripotent stem cells for disease modeling and compound
Payal Chawla1, Niclas Flosdorf2, Marcelo A S de Toledo1
1Department of Medicine IV, Hematology, Oncology and Stem Cell Transplantation, Faculty of Medicine, RWTH Aachen University Hospital, 52074 Aachen, Germany; Center for Integrated Oncology Aachen Bonn Cologne Düsseldorf (CIO ABCD), 52074 Aachen, Germany.
Abstract:
Here, we present a protocol for generating megakaryocytes from patient induced pluripotent stem cells (iPSCs). We describe steps for expanding and dissociating iPSCs into single cells and aggregating them into spin embryoid bodies (EBs). We detail procedures for lineage commitment and differentiation toward megakaryocytes within 14 days. This protocol supports phenotypic characterization and compound testing across multiple patient-derived iPSC lines. For complete details on the use and execution of this protocol, please refer to Flosdorf et al.1.

