RlTTG1 isolated from Rosa laevigata Michx. regulates trichome development and stress response in transgenic...

Area of Science:

• Plant Biology
• Genetics
• Molecular Mechanisms

Background:

• Rosa laevigata Michx. (R. laevigata) possesses significant horticultural and medicinal value, but cultivation is hindered by dense prickles.
• The molecular basis of prickle development in R. laevigata remains largely unexplored.

Purpose of the Study:

• To investigate the molecular mechanism underlying prickle development in R. laevigata.
• To identify genes involved in prickle formation and understand their function in stress response.

Main Methods:

• Transcriptome sequencing to identify prickle-related genes.
• Functional analysis of TRANSPARENT TESTA GLABRA 1 (TTG1) through overexpression in Arabidopsis.
• Physiological and biochemical assays to assess stress tolerance.
• Yeast two-hybrid (Y2H) and Bimolecular fluorescence complementation (BiFC) for protein interaction studies.

Main Results:

• Eighty-one prickle-related genes were identified; RlTTG1 was selected for functional study.
• Overexpression of RlTTG1 in Arabidopsis increased leaf trichome density and enhanced root growth under drought and salt stress.
• RlTTG1 positively regulated proline and superoxide dismutase levels while decreasing malondialdehyde and hydrogen peroxide under stress.
• RlTTG1 was found to interact with several key trichome development regulators.

Conclusions:

• RlTTG1 plays a crucial role in prickle development and abiotic stress tolerance in R. laevigata.
• This study provides a foundation for understanding prickle formation and stress response mechanisms in this species.