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Published on: January 7, 2019
Pulsed ultrasound-assisted deep eutectic solvent-mediated stabilization and bioactivity enhancement of hydrolysable
Xingying Xue1, Zhiwen Qi2, Hao Zhou2
1National Key Laboratory for Development and Utilization of Forest Food Resources, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Nanjing 210042, China; College of Materials Science and Technology, Beijing Forestry University, Beijing 100083, China; International Innovation Center for Forest Chemicals and Materials, Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210037, China.
Abstract:
A pulsed ultrasound-assisted deep eutectic solvent (DES) strategy enabled high-efficiency tannin extraction from chestnut burr waste, yielding 104.40 ± 4.31 mg TAE/g DW-2.1-fold higher than conventional aqueous extraction. Comprehensive mechanistic investigations revealed synergistic effects: DES components enhanced hydrogen bonding and electrostatic attraction with tannins, while a favorable solvation energy barrier (ΔG = -85.15 kcal/mol) facilitated extraction. Advanced spectroscopic analysis (FT-IR, HPLC-IT-TOF-MS2) demonstrated DES-induced structural stabilization of hydrolysable tannins, with key components exhibiting a 3.1-fold increase in relative abundance. Specifically, DES was found to suppress water activity while forming protective tannin-DES complexes, as evidenced by characteristic upfield 1H NMR shifts (Δδ = -0.19 ppm) and distinct vibrational frequency changes. The sustainability of this method was validated through five successful recycling trials and comprehensive green metric assessments. DES-extracted tannins demonstrated >1.2-fold enhanced antioxidant capacity and superior antibacterial activity compared to aqueous extracts. This work establishes an innovative method for agroforestry waste valorization and advances green extraction technologies.
