Composite structure, physicochemical properties, and in vitro digestibility of an ultra-high pressure-induced...

Although it has been demonstrated that proteins can influence the starch digestion process, the specific mechanism by which they act on starch digestion under ultra-high pressure (UHP) treatment conditions remains unclear. In the present study, UHP technology was employed to prepare acetylated chickpea starch (ACPS)-soy protein isolate (SPI) complexes (ACPS-SPI). The mechanism of protein-mediated regulation of starch digestion behavior was systematically investigated through multi-scale structural analysis and digestive property evaluation. The results reveal that UHP treatment significantly regulated the in vitro digestibility of starch. Under a pressure of 500 MPa, the resistant starch (RS) content in acetylated chickpea starch (ACPS) rose from 13.41% to 19.31%. The RS content further increased to 22.52% with the incorporation of SPI, which can be attributed to the combined effects of a continuous physical barrier limiting enzyme access and specific interactions inhibiting enzymatic hydrolysis. Meanwhile, the rapidly digestible starch (RDS) content decreased significantly, while both the slowly digestible starch (SDS) and RS contents exhibited an upward trend. Scanning electron microscopy images reveal that ACPS and SPI form a hybrid morphology with distinct aggregation. X-ray diffraction analysis indicates that the samples retain a C-type crystalline structure, though crystallinity decreases with increasing pressure-ACPS crystallinity drops from 20.35% to 9.08%. Regarding physicochemical properties, starch and its composites exhibit enhanced water-holding capacity at 400 MPa pressure, with system viscosity reaching its maximum value. This study established an efficient method for preparing ACPS-SPI composites, providing a theoretical basis for utilizing UHP and protein synergistic regulation to modulate starch digestion characteristics and delay postprandial glycemic response.