Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bone marrow mesenchymal stem cell-derived TSG-6 regulates microglial pyroptosis and alleviates bone cancer pain by inhibiting the NLRP3 inflammasome.

Stem cell research & therapy·2026

Same author

From agri-food wastes to high-value products: research progress, technological innovation, and challenges in the resource recycling of Chinese Baijiu by-products.

Bioresource technology·2026

Same author

Colloid milling and microfluidization assisted by pH regulation improve the solubility and functional properties of yeast proteins.

Food chemistry·2026

Same author

Nanocellulose Alleviates Intrahepatic Cholestasis of Pregnancy via Gut Microbiota-Mediated Bile Acid Homeostasis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Femtojoule optical Kerr switching with milliwatt-peak-power in silicon-organic hybrid nanocavity.

Nature communications·2026

Same author

An Octopus probe for high-performance >1,300 nm NIR-II fluorescence molecular imaging of cancer.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Sodium-Based Battery Component Design: Imitating Lithium or Forging New Paths?

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Enhancing Birefringence of Sulphates by Polarity Modification in Planar Cations.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

In Situ Atomic-Scale Observation of Preferential Premelting at Oxide Crystal Defects.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Thickness-Dependent Semiconductor-Metal Transition in Two-Dimensional Nonlayered Magnetic CuCo<sub>2</sub>S<sub>4</sub>.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Programmable Control Over Radical and Non‑Radical Pathways in Fenton‑Like Catalysis via Carbon‑Encapsulated Iron Nanoreactors.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Self-Powered MXene@Perovskite Thermoelectric Skin for Multimodal Mid-Infrared Sensing and Human Signal Recognition.

Small (Weinheim an der Bergstrasse, Germany)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 6, 2026

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

In Vivo Microendoscopy in the Near-Infrared II Window.

Zhisheng Wu^1,2, Danyang Xu¹, Zideng Dai¹

¹Department of Electrical and Computer Engineering, School of Biomedical Engineering, The University of Hong Kong, Hong Kong SAR, China.

Small (Weinheim an Der Bergstrasse, Germany)

|March 2, 2026

Summary

This summary is machine-generated.

A new near-infrared II (NIR-II) microendoscopy allows deep intestinal imaging beyond the mucosa in live mammals. This non-invasive technique visualizes rectal vasculature and lymph nodes, aiding early colitis detection.

Keywords:

In vivo imaging colitis lymph node microendoscopy near‐infrared II imaging

More Related Videos

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

Related Experiment Videos

Last Updated: May 6, 2026

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

Area of Science:

Biomedical Engineering
Optical Imaging
Gastroenterology

Background:

Deep tissue fluorescence endoscopy is limited by light scattering, hindering visualization beyond the mucosa.
High spatiotemporal resolution trans-intestinal imaging in live mammals remains a significant challenge.
Current methods often require invasive procedures, limiting longitudinal studies.

Purpose of the Study:

To develop a non-invasive microendoscopy for deep intestinal imaging beyond the mucosa.
To achieve high-resolution, real-time visualization of rectal vasculature and lymphatics.
To enable early detection of intestinal diseases like colitis.

Main Methods:

Development of a near-infrared II (NIR-II) microendoscopy system with imaging wavelengths extended to 1700 nm.
Utilizing sub-10-µm resolution for transrectal imaging in live mammals without surgery.
Employing a large field of view for comprehensive imaging of rectal structures and lumbar lymph nodes.

Main Results:

Successful deep intestinal imaging beyond the mucosa and transrectal imaging at sub-10-µm resolution.
Real-time visualization of vascular networks in different layers of the mouse rectum.
First-time non-invasive imaging of the entire lumbar lymph node, revealing abnormal lymphatic drainage in tumor-bearing mice.
Distinct fluorescence patterns observed in rectal vasculature and lumbar lymph nodes of healthy and colitis-bearing mice, enabling early lesion detection.

Conclusions:

The developed NIR-II microendoscopy overcomes light scattering limitations for deep intestinal imaging.
This technology provides unprecedented non-invasive visualization of rectal microvasculature and lymphatic systems.
It offers new possibilities for studying intestinal microenvironment-lymphatic interactions and early disease diagnosis.