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Identifying Prognostic Biomarkers and Key Pathways in Renal Clear Cell Carcinoma: A Pilot Study Using Integrated
Hamed Manoochehri1, Kosar Mirzaee2, Amir Taherkhani3,4
1The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran, bpums.ac.ir.
Biochemistry Research International
|March 2, 2026
Summary
Specific microRNAs (miRNAs) and hub genes, like the miR-26a-1-3p/RUNX2 axis, show potential as prognostic indicators for renal clear cell carcinoma (RCCC). Understanding these biomarkers and pathways offers new therapeutic avenues for this aggressive kidney cancer.
Area of Science:
- Oncology
- Molecular Biology
- Bioinformatics
Background:
- Renal clear cell carcinoma (RCCC) is an aggressive kidney cancer with a poor prognosis.
- Identifying novel prognostic biomarkers and therapeutic targets is crucial for improving patient outcomes.
- MicroRNAs (miRNAs) and signaling pathways are implicated in RCCC pathogenesis.
Purpose of the Study:
- To identify differentially expressed miRNAs and their target genes in RCCC.
- To assess the prognostic significance of identified miRNAs and hub genes.
- To explore potential therapeutic targets and molecular mechanisms in RCCC.
Main Methods:
- Comparative analysis of miRNA expression profiles from RCCC and normal kidney tissues using bioinformatics.
- Identification of differentially expressed miRNAs (DEMs) and hub genes.
- Validation of key miRNA and gene expression and prognostic value in independent cohorts (GEO, TCGA-KIRC).
- Protein-protein interaction network construction and pathway enrichment analysis.
Main Results:
- Fifteen DEMs and 74 hub genes were identified.
- Downregulation of miR-26a-1-3p, miR-144-3p, and miR-144-5p correlated with poorer RCCC prognosis.
- Overexpression of CDK1 and RUNX2 was validated and associated with decreased patient survival.
- A significant inverse association was found between miR-26a-1-3p and RUNX2 expression.
- Enrichment in PI3K-Akt, MAPK, apoptosis, and cell cycle pathways was observed.
Conclusions:
- The miR-26a-1-3p/RUNX2 axis serves as a potential prognostic indicator in RCCC.
- These findings enhance understanding of RCCC molecular underpinnings.
- Identified biomarkers and pathways offer potential therapeutic opportunities for RCCC.
