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Updated: Mar 3, 2026

Author Spotlight: Evaluating Biophysical Assays for Characterizing PROTACS Ternary Complexes

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Published on: January 12, 2024

PROTAC-Mediated Ternary Complex Stability with Ricin Toxin A: A Computational Perspective.

Fernanda D Botelho¹, Salim T Islam^2,3, Steven R LaPlante^2,3

¹Laboratory of Molecular Modeling Applied to the Chemical and Biological Defense (LMCBD), Military Institute of Engineering, Rio de Janeiro, Rio de Janeiro 22290-270, Brazil.

|March 2, 2026

Summary

This study explores proteolysis-targeting chimeras (PROTACs) as a novel approach to neutralize ricin toxin. Computational models identified promising PROTAC candidates for ricin degradation, offering a new therapeutic strategy.

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A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain RTA Trafficking in Yeast

A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain RTA Trafficking in Yeast

Published on: December 15, 2017

Area of Science:

Biochemistry
Toxicology
Computational Chemistry

Background:

Ricin, a potent toxin from castor beans, lacks approved antidotes or vaccines.
Existing ricin inhibitors and vaccine candidates show limited efficacy, necessitating novel therapeutic strategies.

Purpose of the Study:

To investigate the potential of proteolysis-targeting chimeras (PROTACs) for inducing ricin degradation.
To computationally assess PROTACs' ability to mediate the ubiquitination and proteasomal degradation of ricin toxin.

Main Methods:

Computational study involving molecular docking and molecular dynamics simulations.
Assessment of ricin toxin's catalytic subunit (RTA) stability with E3 ligases VHL and CRBN.
Evaluation of various PROTAC candidates with different linkers for ternary complex formation.

Main Results:

Identified three promising PROTAC candidates (one targeting CRBN, two targeting VHL) with potential for stable ternary complex formation.
Revealed specific PROTAC linkers that may effectively mediate RTA degradation.
Demonstrated the feasibility of PROTAC-mediated degradation of ricin toxin.

Conclusions:

PROTAC-mediated degradation represents a novel therapeutic strategy for ricin intoxication.
The identified PROTAC candidates warrant further in vitro validation.
This research lays the foundation for developing new treatments against ricin poisoning.