Search research articles

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lived experience: from intention to global action.

BMJ (Clinical research ed.)·2025

Same author

More medical journals must work in partnership with patients and the public

BMJ (Clinical research ed.)·2024

Same author

Patients need access to their medical records-now.

BMJ (Clinical research ed.)·2024

Same author

Patient partnership at BMJ.

BMJ (Clinical research ed.)·2023

Same author

Gene expression changes in therapeutic ultrasound-treated venous leg ulcers.

Frontiers in medicine·2023

Same author

Patient advocacy: an antidote to loneliness and more.

BMJ medicine·2023

Same journal

From pixels to length: Body length estimation of aquatic macroinvertebrates from digital images for ecological applications.

MethodsX·2026

Same journal

Sorbent-coated metal discs for time-integrated VOC sampling: A reproducible workflow coupled to SPME-GC/MS.

MethodsX·2026

Same journal

Step-by-step <i>En face</i> O red oil method for aortic plaque staining and quantification in ApoE knockout mouse.

MethodsX·2026

Same journal

Optimized protocols for culturing and sectioning mouse intestinal organoids: enhancing efficiency and structural integrity.

MethodsX·2026

Same journal

MCLF: Montage consistent CNN-Liquid fusion for long-term scalp EEG seizure detection.

MethodsX·2026

Same journal

Facile synthesis of model polystyrene nanoparticles for nanoplastics research.

MethodsX·2026

See all related articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Mar 3, 2026

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

Engineering vascularized 3D tissues: An optimized seeding protocol for collagen-based scaffolds.

Sophia Walker¹, Evelyn Hergenhan¹, Olivia Boerman¹

¹Biomedical Engineering, Bucknell University, Lewisburg, PA, USA.

|March 2, 2026

Summary

This summary is machine-generated.

Optimizing endothelial cell seeding in collagen scaffolds improves 3D culture for physiologically relevant studies. This protocol ensures reproducible, well-connected vascular networks for more reliable in vitro research.

Keywords:

3D culture Angiogenesis Cardiovascular Endothelial Tissue engineering

More Related Videos

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

Related Experiment Videos

Last Updated: Mar 3, 2026

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

Published on: June 16, 2015

Generation of Self-assembled Vascularized Human Skin Equivalents

Generation of Self-assembled Vascularized Human Skin Equivalents

Published on: February 12, 2021

Area of Science:

Biomaterials Science
Cell Biology
Tissue Engineering

Background:

Endothelial cells' sensitivity to mechanical environments limits 2D culture relevance.
3D collagen scaffolds offer more physiologically accurate endothelial cell studies.
Varied seeding protocols hinder reproducible vascularization in collagen scaffolds.

Purpose of the Study:

Optimize endothelial cell seeding for 3D collagen scaffolds.
Achieve reproducible and uniform vascularization.
Enhance the reliability of in vitro endothelial cell studies.

Main Methods:

Developed an optimized protocol for endothelial cell seeding in commercial collagen scaffolds.
Investigated scaffold pre-saturation, seeding strategy, cell density, and incubation time.
Utilized 600,000 cells/100 uL media with opposing-side seeding and 120h incubation.

Main Results:

Achieved reproducibly well-connected vascular networks.
Demonstrated uniform cell distribution throughout the scaffold.
Established a reliable method for vascularizing collagen scaffolds.

Conclusions:

The optimized protocol ensures consistent and interconnected vascular networks in 3D collagen scaffolds.
This method enhances the physiological relevance of in vitro endothelial cell studies.
Provides a standardized approach for researchers using collagen scaffolds.