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MdCSN5-MdIAMT module promotes anthocyanin accumulation by regulating IAA homeostasis in apple
Jiahu Zhang1,2, Chen Wang2,3, Haibo Wang1
1Shandong Institute of Pomology, Taian, Shandong, 271000, China.
Horticulture Research
|March 2, 2026
Summary
Researchers identified a new mechanism for anthocyanin accumulation in apples. The MdCSN5-MdIAMT module regulates auxin homeostasis, impacting anthocyanin biosynthesis and providing insights into plant hormone signaling.
Area of Science:
- Plant Biology
- Molecular Genetics
- Biochemistry
Background:
- Anthocyanin content is crucial for apple breeding.
- Auxin, a plant hormone, influences anthocyanin biosynthesis, but the regulatory mechanism is unclear.
Purpose of the Study:
- To identify key genes regulating apple anthocyanin accumulation.
- To elucidate the molecular mechanism of auxin's role in anthocyanin biosynthesis.
Main Methods:
- Transcriptome sequencing of apple hybrids.
- Gene expression analysis (overexpression in calli and peel).
- Yeast two-hybrid, luciferase complementation imaging, and co-immunoprecipitation assays.
Main Results:
- Identified indole-3-acetic acid (IAA) methyltransferase (MdIAMT) as a key gene.
- Overexpression of MdIAMT increased anthocyanin accumulation by regulating IAA homeostasis.
- Discovered an interaction between MdIAMT and MdCSN5, affecting auxin signaling and promoting anthocyanin accumulation.
Conclusions:
- Elucidated a novel MdCSN5-MdIAMT module regulating auxin homeostasis and anthocyanin accumulation in apples.
- Deepened understanding of plant hormone signaling in anthocyanin biosynthesis.
- Provided a new target for apple breeding and anthocyanin enhancement.
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