Rapid ecotoxicity and genotoxicity assessment using Macropodus ocellatus cells

Area of Science:

• Environmental Toxicology
• Aquatic Toxicology
• Genotoxicity Testing

Background:

• Toxic substances pose significant risks to aquatic ecosystems and human health.
• Simultaneous ecotoxicity and genotoxicity assessment is crucial for detecting potential harm.
• Previous studies established a fish cell system using Cyprinus carpio (C. carpio) for toxicity assessment.

Purpose of the Study:

• To develop and validate a novel ecotoxicity and genotoxicity assessment system using Macropodus ocellatus (M. ocellatus) fish cells.
• To evaluate the efficacy of M. ocellatus cells under conditions previously used for C. carpio cells (1% fetal bovine serum/6 hours).
• To confirm the assessment platform's ability to detect known genotoxic substances.

Main Methods:

• Culturing and treating fish cells derived from Macropodus ocellatus (M. ocellatus).
• Exposing M. ocellatus cells to toxic substances in a medium containing 1% fetal bovine serum (FBS) for 6 hours.
• Analyzing ecotoxicity and genotoxicity data generated from the treated M. ocellatus cells.

Main Results:

• Macropodus ocellatus (M. ocellatus) cells yielded ecotoxicity and genotoxicity data comparable to Cyprinus carpio (C. carpio) cells.
• The M. ocellatus assessment tool successfully identified ecotoxicity and genotoxicity for known harmful substances, including fluxapyroxad, fipronil, and perfluorooctanoic acid.
• The system demonstrated high throughput capacity for analyzing numerous toxins rapidly.

Conclusions:

• An effective ecotoxicity and genotoxicity assessment system using Macropodus ocellatus (M. ocellatus) cells has been established.
• This rapid assessment platform is suitable for analyzing a large volume of toxins within a short timeframe.
• The M. ocellatus cell-based system offers a valuable tool for environmental monitoring and risk assessment.