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Peripheral miRNA-155 Expression and Circulating Inflammatory Cytokines in Patients with Helicobacter
Sarah Kassab Shandaway Al-Zamali1
1Department of Medical Microbiology, Hammurabi College of Medicine, University of Babylon, Hillah, Babylon, Iraq.
Summary
Helicobacter pylori infection elevates systemic miRNA-155 and inflammatory cytokines (IL-6, IFN-γ, IL-10) in chronic gastritis patients. These biomarkers indicate immune dysregulation in peripheral blood.
Area of Science:
- Gastroenterology
- Immunology
- Molecular Biology
Background:
- Helicobacter pylori infection is a primary cause of chronic gastritis and sustained gastric inflammation.
- MicroRNA-155 (miRNA-155) and key inflammatory cytokines are crucial in immune regulation.
- Systemic expression profiles of miRNA-155 and cytokines in H. pylori-associated gastritis require further characterization.
Purpose of the Study:
- To investigate systemic miRNA-155 expression and serum levels of IL-6, IFN-γ, and IL-10 in patients with H. pylori-associated chronic gastritis.
- To determine if these biomarkers differ between patients and healthy controls.
- To explore the association between biomarker levels and H. pylori infection status.
Main Methods:
- A case-control study involving 25 patients with confirmed H. pylori-associated chronic gastritis and 25 age-matched healthy controls.
- Quantitative real-time PCR was used to measure peripheral miRNA-155 expression.
- Enzyme-linked immunosorbent assay (ELISA) quantified serum levels of IL-6, IFN-γ, and IL-10.
Main Results:
- Patients with H. pylori-associated chronic gastritis showed significantly higher peripheral miRNA-155 expression compared to controls (p < 0.001).
- Serum concentrations of IL-6, IFN-γ, and IL-10 were significantly elevated in patients versus controls (all p < 0.001).
- Logistic regression analysis revealed significant associations between the measured biomarker levels and H. pylori infection status.
Conclusions:
- This study reveals significant systemic alterations in miRNA-155 expression and inflammatory cytokines in H. pylori-associated chronic gastritis.
- Findings suggest detectable immune-regulatory changes in peripheral blood.
- Further validation in larger, independent cohorts is recommended to confirm these exploratory findings.
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