Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dextromethorphan-bupropion-associated pharmacovigilance signals based on the FAERS database: An observational study.

Medicine·2026
Same author

Highly selective visual receptive fields in mouse frontal cortex.

bioRxiv : the preprint server for biology·2026
Same author

Qualitative and quantitative assessment of <i>ex vivo</i> human brain tumors using quantitative oblique back-illumination microscopy (qOBM).

Biomedical optics express·2026
Same author

Strong yet ductile heat-resistant aluminum alloy by additive manufacturing.

Nature communications·2026
Same author

Longitudinal, label-free, high-resolution imaging of glioblastoma spheroid response to therapy: a translational tool for preclinical evaluation of chemotherapy, radiation, and immunotherapy.

Optica·2026
Same author

Foundation model cascades enable zero-shot microscopy image analysis for cell therapy manufacturing.

Cytotherapy·2026
Same journal

Physics-guided self-supervised learning for high-fidelity reconstruction in live-cell imaging.

Npj imaging·2026
Same journal

Complementary hyperpolarized <sup>13</sup>C and <sup>15</sup>N MRI reveal divergent signatures of hepatic injury and methyl-donor metabolism.

Npj imaging·2026
Same journal

Optical coherence tomography enables optical biopsy of endometrial tissue for early cancer detection.

Npj imaging·2026
Same journal

My Life as a "Theranostician": A commentary on the evolution of theranostics.

Npj imaging·2026
Same journal

Towards generalisable foundation models for brain MRI.

Npj imaging·2026
Same journal

Reliability of foundation models for image retrieval in histopathology.

Npj imaging·2026
See all related articles

Related Experiment Video

Updated: May 8, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Single capture quantitative oblique back-illumination microscopy.

Paloma Casteleiro Costa1, Srinidhi Bharadwaj2,3, Zhenmin Li2

  • 1School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, GA, USA.

Npj Imaging
|March 2, 2026
PubMed
Summary
This summary is machine-generated.

Single-capture quantitative phase imaging (SCqOBM) uses deep learning to reconstruct 3D images from one capture. This advancement significantly speeds up label-free imaging of biological samples for research and diagnostics.

More Related Videos

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
12:36

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

Related Experiment Videos

Last Updated: May 8, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT)

Published on: August 4, 2018

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
12:36

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Computational Imaging

Background:

  • Quantitative oblique back-illumination microscopy (qOBM) enables label-free, 3D phase imaging of thick biological samples.
  • Traditional qOBM requires multiple captures, limiting imaging speed and system simplicity.

Purpose of the Study:

  • To develop a single-capture qOBM (SCqOBM) method using deep learning for faster, simpler phase recovery.
  • To validate the accuracy and performance of SCqOBM in various biological applications.

Main Methods:

  • Implemented a deep learning model for phase reconstruction from a single oblique back-illumination image.
  • Applied SCqOBM to diverse biological samples, including in-vivo imaging of blood flow.
  • Evaluated imaging speed for single-slice and volumetric refractive index tomography.

Main Results:

  • SCqOBM accurately reconstructs phase information, comparable to traditional four-capture qOBM.
  • Demonstrated non-invasive, in-vivo imaging of blood flow in mouse brain and human arm.
  • Achieved high-speed quantitative phase imaging at 2 kHz and volumetric tomography at 10 volumes/second.

Conclusions:

  • SCqOBM significantly enhances imaging speed and simplifies hardware requirements for quantitative phase imaging.
  • The technique is suitable for dynamic, real-time applications and opens new possibilities in biomedical research and diagnostics.
  • SCqOBM facilitates high-resolution, label-free imaging for non-invasive hematological assessments and in-vivo tissue analysis.