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A Bioluminescence Reporter Mouse Strain for In Vivo Imaging of IFNγ Cell Localization and Function.
Immunology
|March 2, 2026
Summary
We developed a new mouse model to track interferon gamma (IFNγ) in real-time. This tool aids in understanding immune responses in inflammation and cancer, potentially improving immunotherapies.
Area of Science:
- Immunology
- Molecular Biology
- Biotechnology
Background:
- Interferon gamma (IFNγ) is crucial for immune regulation and inflammation.
- Its in vivo dynamics in inflammation and cancer are not fully understood.
Purpose of the Study:
- To develop a novel mouse model for real-time, non-invasive tracking of IFNγ-expressing cells.
- To investigate IFNγ dynamics in inflammatory and carcinogenic conditions.
Main Methods:
- CRISPR/Cas9-mediated homology-directed repair was used to create a C57BL/6J-Ifng-2A-luciferase knock-in mouse strain.
- Luciferase cassette insertion under the endogenous Ifng promoter allows bioluminescence imaging (BLI).
Main Results:
- The model successfully detected transient IFNγ induction by Poly(I:C).
- It enhanced intratumoral IFNγ signals during anti-PD-1/CTLA-4 therapy.
- Dynamic IFNγ expression was tracked during imiquimod-induced psoriasis.
Conclusions:
- This transgenic mouse model offers a powerful tool for longitudinal tracking of IFNγ-expressing cells.
- It provides novel insights into IFNγ-mediated immune regulation in inflammation and cancer.
- The model can help identify IFNγ-related therapeutic targets and predict immunotherapy responses.
