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Evaluation of Intermolecular Interactions in HPLC Using Deuterated Mobile Phases
Kosuke Amano1, Eisuke Kanao2,3, Sayaka Konishi Yamada4
1Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501, Japan.
Analytical Chemistry
|March 3, 2026
Summary
Using deuterated solvents in chromatography significantly alters how molecules interact. This study reveals changes in solute-solvent and solute-stationary phase interactions, impacting retention behavior in analytical and pharmaceutical sciences.
Area of Science:
- Analytical Chemistry
- Physical Chemistry
- Isotope Chemistry
Background:
- Deuterium (heavy hydrogen) is crucial in analytical and pharmaceutical applications.
- The impact of deuterium on intermolecular forces, particularly in chromatography, requires further elucidation.
Purpose of the Study:
- To investigate the effect of solvent deuteration on solute-solvent and solute-stationary phase interactions.
- To analyze how deuterated solvents influence retention behavior in high-performance liquid chromatography (HPLC).
Main Methods:
- Comparison of deuterated and hydrogenated solvents as mobile phases in HPLC.
- Examination of retention behavior in both reversed-phase and normal-phase HPLC modes.
- Utilized a C60-immobilized stationary phase for normal-phase analysis.
Main Results:
- Deuterated solvents increased analyte retention in reversed-phase HPLC, suggesting reduced affinity with hydrophobic compounds due to enhanced solvent-solvent interactions.
- Aromatic analytes showed particularly increased retention, indicating weakened CH-π and OH-π interactions.
- π-conjugated stationary phases exhibited minimal sensitivity to solvent deuteration, implying less affected π-π interactions.
Conclusions:
- Solvent deuteration systematically modifies intermolecular interactions.
- These alterations in interactions are clearly reflected in chromatographic retention behavior.
- Understanding these effects is vital for optimizing analytical and pharmaceutical processes using deuterated solvents.
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