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Published on: April 6, 2016
Three-dimensional patient-derived endometriosis model for drug evaluation
Yuqi Li1, Wei Shao1, Xiaoxiao Gao1
1Department of Obstetrics and Gynecology, Jinshan Hospital of Fudan University, Shanghai, China.
Acta Obstetricia Et Gynecologica Scandinavica
|March 3, 2026
Summary
Researchers developed a novel patient-derived coculture model for endometriosis (EMs). This model revealed heterogeneous responses to dienogest, highlighting interpatient variability in treatment outcomes for this complex gynecological disease.
Area of Science:
- Gynecology
- Cell Biology
- Disease Modeling
Background:
- Endometriosis (EMs) is a complex gynecological disease with unknown pathogenesis and no effective treatments.
- Patient-derived models are essential for understanding disease mechanisms and developing therapeutics.
- Current research focuses on creating in vitro models that mimic the endometriotic microenvironment.
Purpose of the Study:
- To develop a novel coculture system using patient-derived epithelial organoids and stromal cells for endometriosis research.
- To investigate the dynamic interactions between epithelial and stromal cells in the endometriotic microenvironment.
- To assess the therapeutic efficacy of dienogest in a patient-specific endometriosis model.
Main Methods:
- Isolated and cultured epithelial organoids and stromal cells from endometriosis patients.
- Established a coculture system to mimic in vivo pathological features.
- Utilized immunohistochemical assays for biomarker identification and CellTiter-Glo assay for drug response assessment.
Main Results:
- Successfully cultured patient-derived organoids and stromal cells with high consistency to native endometriotic lesions.
- Confirmed comparable expression of key biomarkers (ERs, PRs, E-cadherin, CD44, ICAM1, ITGB3, CK7, MMP2/9, TIMP1, TIMP2) in the models.
- Observed heterogeneous responses to dienogest across patient-derived models, indicating interpatient variability.
Conclusions:
- A novel coculture system integrating epithelial organoids and stromal cells was established to recapitulate the endometriotic microenvironment.
- The patient-derived model provides a more relevant in vitro representation of endometriosis.
- The model demonstrated heterogeneous drug responses to dienogest, suggesting potential for personalized treatment strategies.
