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A guide to selecting high-performing antibodies for STXBP1 (UniProt ID: P61764) for use in western blot,

Riham Ayoubi1, Charles Alende1, Maryam Fotouhi1

  • 1Neurology and Neurosurgery, Montreal Neurological Institute-Hospital, Montreal, Québec, Canada.

F1000Research
|March 3, 2026
PubMed

The Syntaxin-binding protein 1, STXBP1, is a protein involved in docking and fusion of synaptic vesicles, a crucial event for neurotransmitters release into the synapse. Here we have characterized twelve STXBP1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.

Keywords:
MUNC18-1P61764STXBP1Syntaxin-binding protein 1antibody characterizationantibody validationimmunofluorescenceimmunoprecipitationwestern blot

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