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Investigating Intestinal Inflammation in DSS-induced Model of IBD

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Monitoring Biomolecular Changes During Colitis Progression Using Infrared Spectroscopy in Dextran Sodium

Mehmood Pirzada¹, Sachini H Ekanayake¹, Avash Kattel¹

¹Department of Physics and Astronomy, Georgia State University, Atlanta, Georgia, USA.

Journal of Biophotonics

|March 3, 2026

Summary

This summary is machine-generated.

Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy detected stepwise changes in serum biomarkers during colitis progression in mice. This method shows potential for early, minimally invasive monitoring of inflammatory bowel disease.

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Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

Published on: February 14, 2021

Area of Science:

Biomedical Spectroscopy
Inflammatory Bowel Disease Research
Translational Medicine

Background:

Monitoring serum constituents is crucial for assessing inflammation and disease progression.
Inflammatory bowel diseases like colitis require sensitive diagnostic and monitoring tools.

Purpose of the Study:

To evaluate Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy for monitoring biomolecular changes in serum during colitis.
To identify spectral biomarkers indicative of colitis progression in a mouse model.

Main Methods:

A Dextran Sodium Sulfate (DSS) induced mouse colitis model was utilized.
Serum samples were analyzed using ATR-FTIR spectroscopy on days 0, 3, and 7 post-DSS administration.
Changes in lipid, protein, and carbohydrate spectral regions were assessed, focusing on Amide I and Amide II bands.

Main Results:

Stepwise alterations in serum spectral profiles were observed, reflecting changes in major biomolecular classes.
Six spectral biomarkers (SBMs), including ratios related to protein secondary structure, showed statistically significant changes by day 3.
The magnitude of spectral differences increased progressively from day 0-3 to day 3-7 and day 0-7, indicating escalating colitis severity.

Conclusions:

ATR-FTIR spectroscopy can detect sequential biomolecular changes in serum associated with colitis development.
The identified spectral biomarkers show promise for early detection and monitoring of colitis progression.
ATR-FTIR offers a potential minimally invasive pre-screening tool for inflammatory conditions affecting serum composition.