Related Experiment Video
Updated: Jun 12, 2026

Glycan Node Analysis: A Bottom-up Approach to Glycomics
Published on: May 22, 2016
Toward N-Linked Glycoproteins: Three-Step Synthesis of Glycan-Asparagine Conjugates from Unprotected Sugars
Junya Akeno1,2, Kiyosei Takasu1, Kounosuke Oisaki2
1Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan.
None:
We have developed a three-step method to synthesize glycan-asparagine conjugates, a core structural motif of N-linked glycoproteins, directly from unprotected saccharides. This transformation involves preparing carbamoyl radical precursors via anomeric amination and condensation without protection, followed by visible-light-mediated carbamoyl radical addition to dehydroalanine derivatives. Characterized by its broad substrate scope, including sialylated glycans and bioactive peptides, and compatibility with buffered aqueous media, this methodology potentially provides a practical platform for the late-stage glycosylation of proteins.
More Related Videos
11:08Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
08:46Regioselective O-Glycosylation of Nucleosides via the Temporary 2',3'-Diol Protection by a Boronic Ester for the Synthesis of Disaccharide Nucleosides
Published on: July 26, 2018
Related Concept Videos
Protein Glycosylation
Glycosylation occurs in...
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Protein Folding Quality Check in the RER
Proteoglycans
Biosynthesis of Polysaccharides
Peptidoglycan Synthesis