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Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Related Experiment Video

Updated: Mar 18, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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High Coverage Quantitative Lipidomic Analysis for Multiple Biological Matrices Using Ultrahigh-Performance

Qinsheng Chen1, Chenhan Zhang1, Xiaoli Zhang1

  • 1State Key Laboratory of Genetics and Development of Complex Phenotypes, School of Life Sciences, Human Phenome Institute, Zhangjiang Fudan International Innovation Center, Metabonomics and Systems Biology Laboratory at Shanghai International Centre for Molecular Phenomics, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

Analytical Chemistry
|March 3, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a new quantitative lipidomics method using UHPLC-MS/MS for comprehensive lipid analysis. The advanced technique improves separation of acidic lipids and accurately quantifies thousands of lipids across diverse biological samples.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Metabolomics

Background:

  • Lipidome complexity poses challenges for quantification.
  • Limited standards and poor chromatography hinder analysis of acidic lipids.
  • High-coverage lipidomics is crucial for understanding lipid biofunctions.

Purpose of the Study:

  • Develop a reliable, high-coverage quantitative lipidomics method.
  • Improve chromatographic separation for all lipid classes, especially acidic ones.
  • Establish accurate retention time prediction models for lipid subclasses.

Main Methods:

  • Utilized ultrahigh-performance liquid chromatography and tandem mass spectrometry (UHPLC-MS/MS).
  • Employed pH and ammonium gradients for enhanced liquid chromatography (LC) separation.
  • Developed quantitative structure-retention relationship models using 267 lipid standards.
  • Applied multiple-reaction monitoring (MRM) mode for quantification.

Main Results:

  • Achieved improved LC separation for all lipids, particularly acidic ones.
  • Established accurate retention time prediction models (ΔtR < 0.33 min, MRE ∼3.4%).
  • Enabled coverage of over 21,700 lipids in 190 subclasses with high sensitivity, precision, accuracy, and stability.
  • Demonstrated applicability across diverse biological matrices (human plasma, urine, cancer cells, bacteria, plants, liver tissue, feces).

Conclusions:

  • The developed UHPLC-MS/MS method provides high-coverage quantitative lipidomics.
  • This method overcomes previous limitations in lipid analysis, especially for acidic lipids.
  • Offers a powerful tool for investigating lipid functions in health and disease.