Related Experiment Video
Updated: Mar 4, 2026
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
Catalytic Amplification-Mediated Single-Nanotag Assembly for Sensitive Detection of Circulating microRNAs
Xiao Chen1, Chengchao Zhang1, Hui Yin2
1Key Laboratory of Green Chemistry and Technology, Ministry of Education, National and Local Joint Engineering Laboratory for Energy Plant Bio-oil Production and Application, Analytical & Testing Center, Sichuan University, Chengdu 610064, P. R. China.
None:
Circulating microRNAs are promising cancer biomarkers, which are characterized by low abundance, small size, and high sequence homology. As such, enzyme-assisted amplification strategies are often indispensable, which sometimes complicates their detection. Herein, a catalytic enzyme-free single-nanotag assembly strategy is developed for the sensitive quantification of miR-373, thanks to the synergistic amplification effect of catalytic hairpin assembly and single-nanoparticle analysis. Elemental mass spectrometric counting of liberated single nanotags provides a low detection limit of 6.0 amol for miR-373, with validation in human serum and cell lysates.
