Related Experiment Video
Updated: May 2, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
Published on: May 30, 2016
Multimodal Super-Resolution Imaging of Nitrogen-Vacancy Centers via High-Index-Induced Structured Illumination
Kyu Ri Choi1, Mohammed Zia Jalaludeen2, Samuel Begumya2
1Department of Physics, Chungbuk National University, Cheongju, Chungbuk, Republic of Korea.
Abstract:
Structured illumination microscopy (SIM) allows for optical imaging with spatial resolution that surpasses the diffraction limit. To further enhance this super-resolution capability, we present a multimodal approach that integrates SIM with optically detected magnetic resonance (ODMR) of nitrogen-vacancy (NV) centers in diamond-a platform considered one of the most promising platforms for quantum sensing and computing. Both theoretical modeling and experimental results reveal that diamond's high refractive index supports finer structured illumination patterns, thereby accessing higher spatial frequencies and yielding improved resolution. This combined strategy not only enhances NV center localization precision and achieves sub-100-nm resolution but also facilitates spin coherence analysis. Leveraging the high refractive index of diamond in SIM thus offers a powerful pathway toward advancing quantum applications at the nanoscale.
More Related Videos
14:02Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
Published on: October 31, 2020
08:49Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
Published on: December 1, 2023
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Super-resolution Fluorescence Microscopy
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)