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Anticipating mesenchymal stem cell quantification: CD63-Expressing small extracellular vesicles in cord blood serum:
Ali İmran Daştan1, Melek Büyükkınacı Erol2, Büşra Candan Balkan3
1Deparment of Medical Directorate, STEMBIO Cell and Tissue Technology, Kocaeli, Türkiye; Department of Medical Biochemistry, Faculty of Medicine, University of Health Sciences, İstanbul, Türkiye.
Background:
Umbilical cord blood serum (UCB-S) contains small extracellular vesicles (sEVs), which may present valuable information about placental and fetal health. sEVs, microscopic particles secreted by cells, have gained attention in biotechnological research because of their role in cellular communication and their potential as biomarkers. The aim of this study was to investigate the correlation between UCB derived mesenchymal stem cells and expressed CD63 of free sEVs in UCB-S, with the aim of developing a novel biomarker for autologous cord blood banking (A-CBB).
Methods:
In this study, UCB was collected from 10 pregnant volunteers. Mesenchymal stem cells were identified by immunophenotyping analysis. Free sEVs in the UCB-S were detected by nanoparticle tracking analysis and flow cytometric analysis using anti-CD63 antibodies. Correlation analysis was performed to investigate the relationship between nanoparticle size, distribution, CD63 expression in sEVs and the percentage of mesenchymal stem cells in UCB.
Results:
UCB-derived mesenchymal stem cell characterized by CD73, CD105, CD44, CD34 and CD45 expressions were found 99.1%, 99%, 97.8%, 0.4% and 1.1% expression, respectively. Particle size and concentration of sEVs in UCB-S were detected 94 ± 27 nm. 206 × 1010±207 × 109 particle/ml, respectively. A strong positive correlation was found between the mean fluorescence intensity of CD63 expression in UCB-S derived sEVs and the percentage of mesenchymal stem cells in UCB.
Conclusion:
Study findings suggest a potential link between UCB-S derived CD63-expressing sEVs and mesenchymal stem cells in the UCB. This link demonstrated that CD63 in UCB-S derived sEVs could be used as a novel biomarker in A-CBB.
