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Pilot study: predicting the interplay between FOXO1 and its downstream long non-coding RNAs in HCC
Rowan Abdelbary1,2, Sherine K Saber1, Fabian Rose3
1Biotechnology Graduate Program, American University in Cairo, Cairo, Egypt.
Frontiers in Oncology
|March 4, 2026
Summary
Forkhead Box O-1 (FOXO1) acts as a tumor suppressor in liver cancer. This study identified 12 long non-coding RNAs (lncRNAs) regulated by FOXO1, which may mediate its functions in hepatocellular carcinoma (HCC).
Area of Science:
- Molecular Biology
- Cancer Research
- Genetics
Background:
- Forkhead Box O-1 (FOXO1) is a tumor suppressor in hepatocellular carcinoma (HCC), often downregulated with poor prognosis.
- While upstream regulators of FOXO1 in HCC are studied, FOXO1's role in regulating non-coding RNAs, particularly long non-coding RNAs (lncRNAs), is unexplored.
Purpose of the Study:
- To investigate the regulatory role of FOXO1 on long non-coding RNAs (lncRNAs) in hepatocellular carcinoma (HCC).
- To identify potential FOXO1-regulated lncRNAs involved in HCC pathogenesis.
Main Methods:
- Analysis of publicly available ChIP-Seq data from the ENCODE database to identify FOXO1 binding sites on lncRNAs.
- RNA sequencing was performed on Huh-7 cells following FOXO1 knockdown to identify differentially expressed lncRNAs.
Main Results:
- ChIP-Seq analysis identified 982 potential lncRNAs regulated by FOXO1.
- RNA sequencing revealed 131 differentially expressed lncRNAs after FOXO1 knockdown.
- A total of 12 lncRNAs were identified as differentially expressed upon FOXO1 knockdown and possessing a FOXO1 binding site in their promoter region.
Conclusions:
- Twelve lncRNAs were identified as potential downstream targets of FOXO1 in HCC.
- These identified lncRNAs may play a role in mediating FOXO1's tumor-suppressive functions in hepatocellular carcinoma.