Lon protease-mediated secretome profiling in Klebsiella pneumoniae

Lon protease is a global regulator in bacteria affecting biofilm formation, antibiotic resistance, toxin-antitoxin complexes, and proteolysis of damaged proteins. In Klebsiella pneumoniae, Lon has roles in iron response, protein transport, and membrane assembly. Here, we apply bottom-up proteomics to identify proteins in the extracellular environment of WT and Δlon K. pneumoniae.