Protocol for the precise activation of intradental neurons in mice via electrical stimulation

Intradental neurons encode tooth sensation, yet identifying them without sacrificing tooth structural integrity remains challenging. Here, we present a protocol for the non-invasive electrical stimulation of intradental neurons with single-molar tooth resolution in anesthetized mice. We describe details for head fixation to access molars. We then provide steps to deliver direct current (DC) pulses to an individual molar while simultaneously monitoring induced current. The protocol can be combined with neural imaging to identify and characterize intradental sensory responses and circuits. For complete details on the use and execution of this protocol, please refer to Ronan et al.¹.