Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Photoreceptors and Plant Responses to Light

Photoreceptors and Plant Responses to Light

Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Chloroplast photorespiratory bypass in tomato couples carbon-nitrogen assimilation to increase yield and fruit quality.

Cell reports·2026

Same author

CIRBP maintains skeletal muscle mitochondrial energy homeostasis via mt-ATP6 to ameliorate sarcopenia.

Biochemical and biophysical research communications·2026

Same author

Flower-like hierarchical interface engineering for ultra-sensitive simultaneous detection of trace multi-fungicide residues in beverages.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026

Same author

Immune checkpoint inhibitor-related myocarditis in a patient with hepatocellular carcinoma: a case report.

Frontiers in cardiovascular medicine·2026

Same author

Evaluation of Continuous Z-Plasty Technique for Linear Scar Repair on the Face: A Retrospective Analysis.

Advances in skin & wound care·2026

Same author

High-fidelity modular skeletons authenticate a Cambrian origin for Bryozoa.

Nature·2026

Search research articles

Related Experiment Video

Updated: Mar 6, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

SlGRAS17 negatively regulates chlorophyll biosynthesis in tomato.

Jianyong Wang¹, Qingfang Lin¹, Huizhu Yang¹

¹School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.

Plant Physiology

|March 4, 2026

Summary

We identified SlGRAS17 as a negative regulator of chlorophyll in tomato. This transcription factor represses chlorophyll biosynthesis by interacting with SlSEU3 and SlLUG to control SlGLK1 expression.

More Related Videos

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Published on: April 17, 2016

Related Experiment Videos

Last Updated: Mar 6, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Published on: April 17, 2016

Area of Science:

Plant Molecular Biology
Plant Physiology
Agricultural Science

Background:

Chlorophyll content is crucial for photosynthesis and visual quality in tomato (Solanum lycopersicum).
Positive regulators of chlorophyll biosynthesis are well-studied, but repression mechanisms are less understood.
Understanding chlorophyll repression is key to improving tomato crop yield and quality.

Purpose of the Study:

To identify novel negative regulators of chlorophyll biosynthesis in tomato.
To elucidate the molecular mechanism by which SlGRAS17 controls chlorophyll accumulation.
To characterize the interaction of SlGRAS17 with other regulatory factors.

Main Methods:

Isolation and characterization of a high-chlorophyll mutant (hcm1).
CRISPR/Cas9 gene editing to generate slgras17 mutants.
Analysis of SlGRAS17 localization, DNA binding, and protein interactions (co-immunoprecipitation).
Virus-induced gene silencing (VIGS) for genetic analysis.

Main Results:

The GRAS transcription factor SlGRAS17 acts as a negative regulator of chlorophyll accumulation.
slgras17 mutants exhibit dark-green leaves with increased chlorophyll content.
SlGRAS17 directly represses SlGLK1 transcription by binding to its promoter.
SlGRAS17 functions with co-repressors SlSEU3 and SlLUG to form a repressive complex.

Conclusions:

SlGRAS17 is a key component of a novel transcriptional module that represses chlorophyll biosynthesis in tomato.
The SlGRAS17-SlSEU3-SlLUG complex fine-tunes chlorophyll levels by regulating SlGLK1.
This discovery provides new targets for genetic improvement of tomato quality and photosynthetic efficiency.