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Updated: Mar 6, 2026

Conjunctival Commensal Isolation and Identification in Mice
Published on: May 1, 2021
A preliminary investigation on the influence of sample storage on the low-abundant ocular surface microbiome
Jérémy Rotzetter1, Livia Spörri2, Elio L Herzog3
1Department of Ophthalmology, Inselspital, Bern University Hospital, University of Bern, Freiburgstrasse 15, CH-3010 Bern, Switzerland; Interfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, Baltzerstrasse 6, CH-3012 Bern, Switzerland.
Abstract:
In this study, the impact of sample freezing prior to DNA extraction was investigated on a small sample set (n = 6) to determine whether this affects the composition of the ocular surface microbiome - a low biomass environment. Frozen samples exhibited a reduced detection of Gram-negative bacteria compared to directly processed samples.
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