Related Experiment Video

Updated: May 2, 2026

Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein
12:01

Detection of Disease-associated α-synuclein by Enhanced ELISA in the Brain of Transgenic Mice Overexpressing Human A53T Mutated α-synuclein

Published on: May 30, 2015

11.4K

Multiplex amplicon sequencing reveals polyclonality and low macrolide resistance in the French Mycoplasma pneumoniae

Jennifer Guiraud1, Christophe Ginevra2, Cécile Laurier-Nadalié1

  • 1CHU Bordeaux, Bacteriology Department, Bordeaux, France.

International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
|March 4, 2026
PubMed
Abstract

Insights

The 2023-2024 Mycoplasma pneumoniae outbreak in France showed diverse strains, with p1 subtype 2 predominating. Macrolide resistance remains low overall, though some strains showed resistance mutations.

Area of Science:

  • * Medical Microbiology
  • * Infectious Diseases
  • * Genomic Epidemiology

Background:

  • * Global surge in Mycoplasma pneumoniae infections noted since late summer 2023.
  • * Previous reports from Southeast Asia indicated p1 subtype 1 predominance and high macrolide resistance.
  • * Limited European data on circulating M. pneumoniae strains and resistance patterns during this outbreak.

Purpose of the Study:

  • * To characterize Mycoplasma pneumoniae strains prevalent in French hospitalized patients during the 2023-2024 outbreak.
  • * To investigate the genetic diversity and antibiotic resistance profiles of circulating strains.
  • * To utilize high-throughput amplicon sequencing for detailed strain analysis.

Main Methods:

  • * Phylogenetic analysis employing p1 adhesin gene subtyping and multi-locus sequence typing (MLST).
  • * Screening for antibiotic resistance-associated mutations in key genes (23S rRNA, 16S rRNA, rplD, rplV, gyrA, parC).
  • * Analysis of clinical data, including respiratory and extrapulmonary manifestations.

Main Results:

  • * Identified five p1 subtypes and 29 distinct sequence types (STs), with p1 subtype 2 (67.2%) and ST14 (29.7%) being most frequent.
  • * P1 subtype 2 was more common in adults, while subtype 1 was linked to extrapulmonary manifestations (5.5% of cases).
  • * Low prevalence (3.0%) of macrolide resistance-associated mutations observed, with a trend towards subtype 1 and mixed populations; no fluoroquinolone or tetracycline resistance mutations detected.

Conclusions:

  • * The 2023-2024 Mycoplasma pneumoniae outbreak in France involved polyclonal strain spread.
  • * A low prevalence of macrolide resistance was documented in the studied French population.
  • * Further surveillance is needed to monitor evolving resistance patterns and strain dynamics.
Keywords:
Community-acquired pneumoniaGenotypingMacrolide resistanceMycoplasma pneumoniaeNGSOutbreak

More Related Videos

Nanothermite with Meringue-like Morphology: From Loose Powder to Ultra-porous Objects07:46

Nanothermite with Meringue-like Morphology: From Loose Powder to Ultra-porous Objects

Published on: December 24, 2017

8.2K
Three-Dimensional Ultrasonic Needle Tip Tracking with a Fiber-Optic Ultrasound Receiver04:33

Three-Dimensional Ultrasonic Needle Tip Tracking with a Fiber-Optic Ultrasound Receiver

Published on: August 21, 2018

10.9K

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
836
Atypical Pneumonia01:14

Atypical Pneumonia

Atypical pneumonia, often caused by Mycoplasma pneumoniae, is a form of pulmonary infection that differs from the classical presentation of bacterial pneumonia in both its cause and clinical symptoms. Mycoplasma pneumoniae is a pleomorphic bacterium notable for its lack of a rigid cell wall. This structural characteristic imparts resistance to beta-lactam antibiotics and significantly influences the bacterium’s behavior within the human host.Other pathogens responsible for the disease...
84
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and...
219
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within...
90