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Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
Published on: February 5, 2014
A trio-binned, haplotype-resolved genome sequence of the zebrafish, Danio rerio Hamilton 1822, SAT strain
Kerstin Howe1, Arang Rhie2, Sergey Koren2
1Wellcome Sanger Institute, Hinxton, England, UK.
Abstract:
We present the trio-binned, haplotype-resolved genome assemblies (released in 2020) of both haplotypes of an individual of the Danio rerio SAT strain, a cross between Tuebingen (maternal) and AB (paternal) strains (zebrafish; Chordata; Actinopteri; Cypriniformes; Cyprinidae). The genome sequence of the paternal haplotype (fDreABH1) is 1,354.1 megabases long, while the genome sequence of the maternal haplotype (fDreTuH) is 1,360.5 megabases long. Most of the assembly is scaffolded into 25 chromosomal pseudomolecules. The mitochondrial genome has also been assembled and is 16.6 kilobases in length.
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