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A Neural Network-Based Identification of Developmentally Competent or Incompetent Mouse Fully-Grown Oocytes
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Inverted microscopy as a high performance tool for pre denudation evaluation of oocyte nuclear maturity
Nuri Peker1, Ayşen Yücetürk1, Özge Karaosmanoğlu1
1Acıbadem Maslak Hospital, In Vitro Fertilization Center, İstanbul, Türkiye.
Turkish Journal of Obstetrics and Gynecology
|March 5, 2026
Summary
An inverted microscope accurately predicts oocyte nuclear maturation before cumulus-oocyte complex (COC) denudation. This method offers high diagnostic accuracy, potentially improving in vitro maturation outcomes.
Area of Science:
- Reproductive Biology
- In Vitro Fertilization
- Oocyte Assessment
Background:
- Accurate oocyte nuclear maturation assessment is crucial for IVF success.
- Current methods lack definitive pre-denudation evaluation.
- Cumulus-oocyte complex (COC) communication is vital for maturation.
Purpose of the Study:
- To evaluate the inverted microscope as a reliable method for assessing oocyte nuclear maturation.
- To determine the diagnostic accuracy of inverted microscopy before COC denudation.
Main Methods:
- Prospective observational study involving 250 COCs from follicles <10 mm.
- Oocytes classified as germinal vesicle (GV) or non-GV using an inverted microscope.
- Diagnostic accuracy assessed after COC denudation and confirmation.
Main Results:
- Inverted microscopy achieved 96.91% accuracy for GV oocytes and 97.64% for non-GV oocytes.
- High concordance between pre- and post-denudation assessments.
- No significant difference in accuracy among embryologists.
Conclusions:
- Inverted microscopy provides a highly accurate method for predicting oocyte nuclear maturation.
- This technique can potentially enhance in vitro maturation by preserving COC communication.
- Enables informed decisions prior to oocyte denudation.
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